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EasyFusion Halo

Plasmid

#112850

Purpose

General donor vector with HaloTag coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

HaloTag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

No

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pimEJ5GFP

Plasmid

#44026

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

Insert

EJ5GFP egfp-based chromosomal break reporter

Use

Tags

Expression

Mammalian

Mutation

pCAGGS promoter and eGFP separated by pgkPURO cas…

Promoter

pCAGGS

Available since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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K44A HA-dynamin 1 pcDNA3.1

Plasmid

#34683

Depositor

Sandra Schmid

Insert

dynamin 1ba (DNM1L Human)

Use

Tags

HA (variant)

Expression

Mammalian

Mutation

K44A

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pET28a-SENP1 (catalytic domain)

Plasmid

#16356

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP1 catalytic domain (SENP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Amino Acid 419 to 644 catalytic domain of human S…

Promoter

Available since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pmGFP10C-Tau

Plasmid

#71433

Purpose

Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71434

Depositor

Henri Huttunen

Article

Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

Insert

Tau (0N4R) (MAPT Human)

Use

Tags

GFP (1-212)

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only
pATetO 6XHis-Taq DNA Ligase

Plasmid

#178798

Purpose

Inducible expression of Taq DNA Ligase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Ligase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry

Plasmid

#108685

Purpose

Strong CAG promoter with mCherry expression for transfection efficacy screening

Depositor

Jordan Green

Article

Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI4

Plasmid

#42528

Depositor

James Hurley

Article

Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

Insert

WIPI4 (WDR45 Human)

Use

Tags

GST-TEV

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-GCaMP5G

Plasmid

#31788

Purpose

a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

Depositor

Loren Looger, GENIE Project, Douglas Kim

Article

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

GCaMP5G

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV_AncBE4max

Plasmid

#112094

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

AncBE4max

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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