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pET21b-LOV-ipaA L514K L531E

Plasmid

#40253

Depositor

Brian Kuhlman

Article

Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

Insert

LOV-ipaA L514K L531E

Use

Tags

6xHis

Expression

Bacterial

Mutation

L514K; L531E; Jα helix region hybridized (AsLOV2 …

Promoter

T7

Available since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
TFORF0544

Plasmid

#141641

Purpose

Lentiviral vector for overexpressing the ZFAT transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZFAT (ZFAT Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-CBX1 Chromo

Plasmid

#59697

Purpose

Contains histone modification interacting domain (CBX1 Chromo)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

CBX1 Chromo (CBX1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)

Plasmid

#104568

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

dLbCpf1(D832A)-DmrA(x1)

Use

Tags

3x FLAG, 3x HA, and NLS

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2)

Plasmid

#104569

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domains

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

dLbCpf1(D832A)-DmrA(x2)

Use

Tags

3x FLAG, 3x HA, and NLS

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU38.2-2.2

Plasmid

#110670

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMiR-RECK-3'UTR-miR-7-mut

Plasmid

#53692

Purpose

Luciferase reporter assay for RECK 3'UTR that has point mutations on miR-7 binding site

Depositor

Edward Chan

Article

Jung et al J Biol Chem. 2012 Aug 24;287(35):29261-7

Insert

RECK-3'UTR (RECK Human)

Use

Luciferase

Tags

Expression

Mutation

nucleotide position 171-174 are mutated to GAAG

Promoter

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
integrin alpha9 W999A pcDNA1 neo

Plasmid

#13589

Depositor

Dean Sheppard

Article

Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

Insert

integrin alpha 9 W999A (ITGA9 Human)

Use

Tags

Expression

Mammalian

Mutation

W999A

Promoter

Available since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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TFORF2387

Plasmid

#144019

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MBD2 (MBD2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF1674

Plasmid

#142369

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

RUNX1T1 (RUNX1T1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF1953

Plasmid

#142897

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PARP1 (PARP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF1676

Plasmid

#143832

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

RUNX1T1 (RUNX1T1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF1928

Plasmid

#142291

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

XRCC5 (XRCC5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF0356

Plasmid

#143695

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF33A (ZNF33A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF0027

Plasmid

#143682

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF44 (ZNF44 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF0635

Plasmid

#143721

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TRIM33 (TRIM33 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF0028

Plasmid

#143683

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF44 (ZNF44 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF1035

Plasmid

#143746

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

WT1 (WT1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF3006

Plasmid

#144482

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZBTB12 (ZBTB12 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF3002

Plasmid

#144478

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NFYC (NFYC Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge