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pDONR_P2R-P3_3'arm_apobb.1

Plasmid

#128681

Purpose

apobb.1 homology arm 3' gateway clone

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

apobb.1 (apobb.1 Zebrafish)

Use

Multi-step gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag YFP hNKCC1 L717C C723S C724V (NT531)

Plasmid

#50896

Purpose

Expresses human NKCC1 L717C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

Depositor

Biff Forbush

Insert

human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)

Use

Tags

3x Flag and YFP

Expression

Mammalian

Mutation

L717C C723S C724V in hNKCC1 in NT17

Promoter

CMV

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_PGKY_APikL3

Plasmid

#123652

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

PGKY_APikL3

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_CD156_APikL3

Plasmid

#123651

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

CD156_APikL3

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_BR32_APikL2

Plasmid

#123649

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

BR32_APikL2

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_BR32_APikL3

Plasmid

#123650

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

BR32_APikL3

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pFH100

Plasmid

#128205

Purpose

improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH101

Plasmid

#128206

Purpose

sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL50014

Plasmid

#50313

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal HBP tag (HA-PreScission-Biotin)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry pBSDONR P4r-P2

Plasmid

#121528

Purpose

For making C-terminal mCherry fusions using multisite Gateway cloning

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2019

Availability

Academic Institutions and Nonprofits only

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LIIc F 1-2 RNAi

Plasmid

#54402

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pDD401

Plasmid

#91828

Purpose

Pmyo-2::GFP FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Pmyo-2::GFP

Use

CRISPR

Tags

Expression

Worm

Mutation

Nucleotide 489 of the myo-2 promoter was changed …

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pOGG203

Plasmid

#115504

Purpose

Clone of a level 1 unit which encodes a constitutively-expressed mCherry

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

pL1M-F2-pNeo pOGG001, mCherry EC15071, T-pharma(pOGG003

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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BASIC_10_J23101-RBS32-GFP-B0015

Plasmid

#68144

Purpose

storage vector for BASIC part: GFP expression cassette

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

J23101-RBS32-GFP-B0015

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF1C09

Plasmid

#70834

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MRG15 (MRG15 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pCR2.1-ZFpparg

Plasmid

#44151

Purpose

This is intended for use in riboprobe synthesis, not for cloning.

Depositor

John Rawls

Article

Flynn EJ et al J Lipid Res. 2009 Aug;50(8):1641-52. doi

Insert

Peroxisome proliferator-activated receptor gamma (pparg Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only
TALEN-ID12-T

Plasmid

#41479

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Use

Tags

3xFLAG and WT FokI nuclease domain

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF1E08

Plasmid

#70829

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Cdk9 (Cdk9 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
Human NCKX4-AS-short (pcDNA3.1+)

Plasmid

#75210

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Article

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (SLC24A4 Human)

Use

Tags

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF1G09

Plasmid

#70837

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

TfIIEalpha (TfIIEalpha Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only