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Plasmid#220848PurposeLentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFSDepositorInsertZFcharm Kv2
UseLentiviralTagsP2A-mCherryExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBMN-AS-HNF4α
Plasmid#139305PurposeKnocking down of human HNF4α through shRNA and amiRNA targeting HNF4αDepositorInsertshRNA and amiRNA against HNF4α
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Chr7_Centromere-Targeting_gRNA
Plasmid#195129Purposedual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFPDepositorInsertChr7 gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-AtU6-29
Plasmid#66203Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-Rabin8
Plasmid#118070PurposeExpresses Flag-tagged human Rabin8 in mammalian cellsDepositorInserthuman Rabin8 (RAB3IP Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB402
Plasmid#74796PurposeGateway cloning compatible binary vector for expression of gene by CaMV35S promoter.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
WT-eGFP
Plasmid#215168PurposeEncodes the enhanced green fluorescent proteinDepositorInsertWT-eGFP
UseTagsHis-tagExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNCS-CyOFP
Plasmid#74278PurposeConstitutive expression of CyOFP in bacteriaDepositorInsertCyOFP
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-GFAP(long)-FLEX-GCaMP7f
Plasmid#189612PurposeCre-dependent GCaMP7f under the GFAP promoterDepositorInsertGCaMP7f
UseAAV and Cre/LoxTagsGCaMP7fExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
xCas9 3.7
Plasmid#108379PurposeMammalian expression vector for xCas9 3.7DepositorInsertxCas9 3.7
UseTagsNLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP Snail WT
Plasmid#16225DepositorInsertSnail (SNAI1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKmK.T5
Plasmid#125057PurposeStorage plasmid for K.marxianus terminator; type 4 partDepositorInsertPGK1 terminator
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Luciferase
Plasmid#99283PurposeAll-in-one lentivector with tet-inducible luciferaseDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
jk95
Plasmid#120176PurposeCAG:gfp:P2A:mycNRNXDepositorInsertCAG:gfp:P2A:myc_NRXN
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-crRNA
Plasmid#213753PurposeFor circular RNA-mediated prime editor using LbCas12a-crRNA in HEK293T cellsDepositorInsertLbCas12a-crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.tRFP
Plasmid#57826PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-tRFP
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab27A
Plasmid#89237PurposeExpression of GFP-tagged human Rab27A in mammalian cellsDepositorInserthuman Rab27A (RAB7A Human)
UseTagseGFPExpressionMammalianMutationwild typePromoterCMVAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P16
Plasmid#125049PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertFBA1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A-YFP-SLBP
Plasmid#205762PurposeSingle-color FUCCI4 senor for transitions of all four cell cylce phasesDepositorInsertrsGFE-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-SK62A-2A-YFP-SLBP
UseLentiviralTagsExpressionMammalianMutationwtPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166
Plasmid#109328PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1 1-150
Plasmid#24389DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationContains amino acids 1-150.PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
TFORF2885
Plasmid#144256PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGLI2 (GLI2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TEK-V5/HIS
Plasmid#205611PurposeExpression of human TEK receptor tyrosine kinase in mammalian cellsDepositorInsertTEK receptor tyrosine kinase (TEK Human)
UseTagsV5-HisExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHDM-SARS-CoV-2 Δ18
Plasmid#164434PurposeEncodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 Spike truncated to remove 18 amino acids (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11
Plasmid#211942Purposedual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterHSP68Available sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P7
Plasmid#125040PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertTEF1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
Plasmid#207863PurposeAAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNADepositorInsertNpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB1619 hU6-BpiI-Cas13bt1-DR
Plasmid#176314PurposehU6-BpiI-Cas13bt1-DR (crRNA expression)DepositorInsertCas13bt1 crRNA direct repeat
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp_CAG:EYFP
Plasmid#51791PurposeConstitutively expresses yellow fluorescent protein , EYFPDepositorInsertEYFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWPI/hPLK2WT/Neo
Plasmid#35385DepositorInserthPLK2 (PLK2 Human)
UseLentiviralTagsEMCV IRES-Neomycin cassette and V5 epitope and Po…ExpressionMammalianMutationPromoterEF1-alphaAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-2200
Plasmid#31062DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutation-2200 to -1 of P2 HNF4A promoterPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Blast DEST (w524-1)
Plasmid#19065Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, BlastDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Calnexin-C-14
Plasmid#54020PurposeLocalization: Endoplasmic Reticulum, Excitation: 487, Emission: 509DepositorInsertCalnexin (CANX Human)
UseTagsmEmeraldExpressionMammalianMutationI474N in CalnexinPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYLsgRNA-AtU3d
Plasmid#66200Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAtU3dAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/opto-a1AR-EYFP
Plasmid#20947PurposeMammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signalingDepositorInsertopto-a1AR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
RIB VP64 R1
Plasmid#161493PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting R1 in the RIB1 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-PBZ-VN
Plasmid#110646Purposeexpresses a PAR-binding (PBZ) domain fused to venus N-terminusDepositorInsertPBZ domain from APLF, amino acids 371-451 (APLF Human)
UseTagsDDK and VNExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-PBZ-VC
Plasmid#110648Purposeexpresses a PAR-binding (PBZ) domain fused to venus C-terminusDepositorInsertPBZ domain from APLF, amino acids 371-451 (APLF Human)
UseTagsHA and VCExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHN781
Plasmid#178828PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
UseTagsHis8, TEVExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-iRFP720
Plasmid#89687PurposepCAG promoter driving iRFP720DepositorInsertiRFP720
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only