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Plasmid#50476PurposeGq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoterDepositorUseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only
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pIIIA/MS2-2
Plasmid#220632PurposeFor expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBA-DO(FAS)-GCaMP6s
Plasmid#110135PurposeCre-off expression of GCaMP6sDepositorInsertGCaMP6s
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLG010 (DRT type 2)
Plasmid#157888PurposeExpresses the DRT type 2 defense systemDepositorInsertDRT type 2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEAK-Flag/INPP4B
Plasmid#24324DepositorInsertInositol Polyphosphate 4-Phosphatase Type II (INPP4B Human)
UseTagsFlag and HAExpressionMammalianMutationnonePromoterAvailable sinceMay 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
NLS-mCherry-NES reporter (pDN160)
Plasmid#72660PurposeNLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158DepositorInsertNLS-mCherry-NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-lck-jGCaMP8f
Plasmid#176759PurposeAAV-mediated expression of membrane bound GCaMP8f under the control of GfaABC1D promoterDepositorHas ServiceAAV1 and AAV9Insertlck-jGCaMP8f
UseAAVTagsExpressionMammalianMutationPromoterGfaABC1DAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYTK072
Plasmid#65179PurposeEncodes ConRE as a Type 5 part to be used in the Dueber YTK systemDepositorInsertConRE
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
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AAVS1-Neo-TRE-CMV-Cre-rtTA
Plasmid#165457PurposeTetracycline inducible CRE expression from AAVS1 locusDepositorInsertCRE
UseTagsExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynaptoTAG2
Plasmid#175275PurposeAAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.DepositorInsertEGFP-Synaptobrevin-2; tdTomato (Vamp2 )
UseAAVTagsEGFP and tdTomato (P2A cleavage)ExpressionMutationPromoterSynapsinAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP-150TAG
Plasmid#174076PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,DepositorInsertSuperfolder GFP
UseTagsHis-6ExpressionBacterialMutationN150TAGPromoterT7Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pGMC00022 (aka pRC0433)
Plasmid#195321PurposeLentiviral expression vector derived from LEGO-G2 with hygromycin selectionDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-J chain
Plasmid#145146PurposeMammalian expression plasmid for J chain of polymeric antibody classesDepositorInsertimmunoglobulin J chain (JCHAIN Human)
UseTagsExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV tat-P2A-rev
Plasmid#101340PurposeLTR drives GFP and internal SFFV promoter drives codon optimized tat and rev.DepositorInsertGFP, tat, rev
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-BFP
Plasmid#64323PurposeExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptideDepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-EBFP2ExpressionMammalianMutationPromoterCBh and U6Available sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-tetO-NGN2
Plasmid#197090PurposePiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiationDepositorInsertNGN2 (NEUROG2 Human)
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRMT8-FLAG
Plasmid#164699PurposeExpression of PRMT8 with a C-terminal FLAG tagDepositorInsertPRMT8 (PRMT8 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
35S-eGFP-nosT
Plasmid#80127PurposeTransient expression vector for eGFP in plantsDepositorTypeEmpty backboneUseTagseGFPExpressionPlantMutationPromoterCaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH2.203_PB-EPN24-MCP-IRES-eGFP-PGK-Blast
Plasmid#205555PurposeExpress EPN24-MCP-IRES-eGFP and blasticidin resistance markerDepositorInsertEPN24-MCP
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pylT-AznLRS
Plasmid#172482PurposeFor E.Coli expression, 306L309L348I384F411WDepositorInsertAznLRS and pylTcua
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
p50 cFlag pcDNA3
Plasmid#20018DepositorInsertNfkb1 (Nfkb1 Mouse)
UseTagsFlagExpressionMammalianMutationTruncated at the p105 ligation site. It codes for…PromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTMiniTrex-mCherry-Shark2
Plasmid#233144PurposeServes as a template plasmid for amplifying Shark2 (Tet-ON) hammerhead aptazyme-3xTy-tagging cassetteDepositorInsertsmCherry
Shark2
UseProkaryotic expressionTags3x Ty tagExpressionMutationCodon optimized for Trypanosoma cruziPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
MIC60-mCherry
Plasmid#232789PurposeEncodes an mCherry-tagged MIC60/IMMTDepositorInsertMICOS complex subunit MIC60 (IMMT Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
BIC-Gag-DDX3
Plasmid#233687PurposeExpresses a fusion between the Murine Leukemia Virus Gag polyprotein and the human DDX3 protein to produce virus-like particles loaded with DDX3 and deliver it to target cells.DepositorInsertDEAD-box helicase 3 (DDX3X Human)
UseRetroviralTagsGag (Murine Leukemia Virus)ExpressionMammalianMutationPromoterhCMVAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDP0437
Plasmid#221127PurposePlasmid for generating integration cassette to C-terminally tag gene of interest with pHluorin::HPHMXDepositorInsertpHluorin
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMVtrunc Affimer6-msfGFPΔN12
Plasmid#231550PurposeMammalian expression of Affimer6 fused to N-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopyDepositorInsertAffimer6
UseTagsmsfGFPExpressionMammalianMutationPromoterCMVtruncAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP12068 - pAAV-DLX2.0-minBG-ChrimsonR-tdTomato-WPRE3-BGHpA (Alias: CN2068)
Plasmid#229775Purposeenhancer AAV for expression of ChrimsonR-tdTomato under the DLX2.0 enhancerInsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHPHS232
Plasmid#228356PurposeLanding pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locusDepositorInsertsmTagBFP2
Reverse tetracycline transactivator 3
neo
UseTagsBlasticidin S deaminase and iCasp9ExpressionMammalianMutationPromoterTRE3GV and gene trapAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-enCjCas9-BlastR
Plasmid#220978PurposeExpresses human codon-optimized enCjCas9DepositorInsertenCjCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-SlugCas9-BlastR
Plasmid#220965PurposeExpresses human codon-optimized SlugCas9DepositorInsertSlugCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV099
Plasmid#215305PurposeExpresses sfGFP under induction with the Cuminic Acid Marionette Cassette (CymRAM + PCymRC-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPCymRCAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
mouse a2b full length
Plasmid#217812PurposeFull length mouse integrin a2b expressionDepositorInsertITGA2B (Itga2b Mouse)
UseTagsP2A-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA_662
Plasmid#216101PurposeCRISPRi, EFS-driven dCas9-KRAB (ZNF10) (Cas only)DepositorInsertCas9 [Sp]
UseCRISPR and Lentiviral; Assembled vectorTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA_831
Plasmid#216100PurposeCRISPRa, EF1a-driven dCas9-VP64 (Cas only)DepositorInsertCas9 [Sp]
UseCRISPR and Lentiviral; Assembled vectorTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pORF1-2
Plasmid#206024PurposeAn expression vector for mouse LINE1 ORF1-2DepositorInsertORF1-2 derived from mouse LINE1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_TBL1XR1:1-514
Plasmid#211167PurposeMammalian expression of full-length human TBL1XR1. C-terminal HiBiT tag.DepositorInsertTBL1XR1:M1-K514 (TBL1XR1 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHelper_TetR/pTet-Tnpase (camR)
Plasmid#213910PurposeATc-inducible himar1C9 transposase expression - cam resistanceDepositorInsertTetR/ptet ATc-inducible himar1C9 transposase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only