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Plasmid#215846PurposeExpression of CXCR4 target with mismatch (MM) 4_DepositorInsertCXCR4 target with mismatch (MM) 4x
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK1-CXCR4
Plasmid#215845PurposeExpression of CXCR4 target with perfect-match siteDepositorInsertCXCR4 target with perfect-match site
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG/HA-p54
Plasmid#215844PurposeExpression of FLAG/HA-p54DepositorInsertHuman p54
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRT
Plasmid#214075PurposeLuminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSK-Spsg
Plasmid#214351PurposeExpresses cloning backbone for SpCas9 sgRNADepositorInsertSpCas9 tracrRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSK-Sasg
Plasmid#214350PurposeExpresses cloning backbone for SlugCas9 sgRNADepositorInsertSlugCas9 tracrRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRT_RTctrl
Plasmid#214078PurposeLuminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRTi
Plasmid#214076PurposeLuminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRTX_ctrl
Plasmid#214077PurposeLuminescent reporter system, NMD negative control. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationRemnant of linker sequence present at the end of …PromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHB1
Plasmid#204668PurposepUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructsDepositorInsertFRT-erm-FRT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA2.0
Plasmid#208299PurposeExpresses ABE-RA2.0 in mammalian cellsDepositorInsertTadA-RA2.0-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA3.0
Plasmid#208301PurposeExpresses ABE-RA3.0 in mammalian cellsDepositorInsertTadA-RA3.0-SpCas9 D10A
UseTagsExpressionMammalianMutationE27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA3.1
Plasmid#208302PurposeExpresses ABE-RA3.1 in mammalian cellsDepositorInsertTadA-RA3.1-SpCas9 D10A
UseTagsExpressionMammalianMutationE27D, R47K, P48A, R51H, I76F, D108G, K110R, H122R…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA3.2
Plasmid#208303PurposeExpresses ABE-RA3.2 in mammalian cellsDepositorInsertTadA-RA3.2-SpCas9 D10A
UseTagsExpressionMammalianMutationE27D, P48A, R51H, D108G, K110R, A114V, H122R, M12…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA3.3
Plasmid#208304PurposeExpresses ABE-RA3.3 in mammalian cellsDepositorInsertTadA-RA3.3-SpCas9 D10A
UseTagsExpressionMammalianMutationE27D, R47K, P48A, R51H, I76F, D108G, K110R, A114V…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.0
Plasmid#208305PurposeExpresses ABE-RA4.0 in mammalian cellsDepositorInsertTadA-RA4.0-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH1-CMV-FIP200-V44D/V314D-mCherry-Hygro
Plasmid#210855PurposeLentiviral expression vector encoding FIP200 mutant-mCherryDepositorInsertFIP200 (RB1CC1 Human)
UseLentiviralTagsmCherryExpressionMutationV44D/V314DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH1-CMV-FIP200-V314D-mCherry-Hygro
Plasmid#210854PurposeLentiviral expression vector encoding FIP200 mutant-mCherryDepositorInsertFIP200 (RB1CC1 Human)
UseLentiviralTagsmCherryExpressionMutationV314DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH1-CMV-FIP200-V44D-mCherry-Hygro
Plasmid#210853PurposeLentiviral expression vector encoding FIP200 mutant-mCherryDepositorInsertFIP200 (RB1CC1 Human)
UseLentiviralTagsmCherryExpressionMutationV44DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TSF-ATG101
Plasmid#210858PurposeMammalian expression of ATG101DepositorInsertATG101 (ATG101 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only