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pDONR223_C19orf48_p.L70F

Plasmid

#81288

Purpose

Gateway Donor vector containing C19ORF48 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

C19ORF48 (C19orf48 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

L70F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPANXN4_p.P4Q

Plasmid

#81262

Purpose

Gateway Donor vector containing SPANXN4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPANXN4 (SPANXN4 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

P4Q

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PDZD11_p.R133H

Plasmid

#81264

Purpose

Gateway Donor vector containing PDZD11 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PDZD11 (PDZD11 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R133H, R137W

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_REG1A_p.S131I

Plasmid

#81265

Purpose

Gateway Donor vector containing REG1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

REG1A (REG1A Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S131I

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RHOA_p.G62E

Plasmid

#81268

Purpose

Gateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RHOA (RHOA Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G62E

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KLRC2_p.G174R

Plasmid

#81270

Purpose

Gateway Donor vector containing KLRC2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KLRC2 (KLRC2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

N2S, F102S, G174R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPATA8_p.E18K

Plasmid

#81272

Purpose

Gateway Donor vector containing SPATA8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPATA8 (SPATA8 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E18K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAB42_p.A61T

Plasmid

#81273

Purpose

Gateway Donor vector containing RAB42 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAB42 (RAB42 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

A61T

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPATA8_p.P83T

Plasmid

#81260

Purpose

Gateway Donor vector containing SPATA8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPATA8 (SPATA8 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

P83T

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RHOA_p.Y42S

Plasmid

#81261

Purpose

Gateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RHOA (RHOA Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Y42S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF2F04

Plasmid

#70901

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

mod(mdg4) (mod(mdg4) Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pgLAP1

Plasmid

#19702

Depositor

Peter Jackson

Type

Empty backbone

Use

Tags

EGFP, S peptide, and TEV

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2009

Availability

Academic Institutions and Nonprofits only

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pgLAP2

Plasmid

#19703

Depositor

Peter Jackson

Type

Empty backbone

Use

Tags

Flag, S peptide, and TEV

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2009

Availability

Academic Institutions and Nonprofits only

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pTol2pA2-mnx1:MLS-KillerRed

Plasmid

#115515

Purpose

Plasmid driving expression of membrane bound KillerRed fluorophore in zebrafish motor neurons

Depositor

Marco Morsch

Article

Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

Insert

MLS-KillerRed

Use

Gateway cloning

Tags

MLS

Expression

Mutation

Promoter

-3mnx1

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_TOP-NLuc1.1_L5-L4

Plasmid

#113867

Purpose

Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

TOP (wnt dependent promoter)
NLuc

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-U6:gRNA

Plasmid

#63156

Purpose

Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.

Depositor

Leonard Zon

Article

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Type

Empty backbone

Use

CRISPR; Tol2 destination vector for gateway

Tags

Expression

Mutation

Promoter

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR4_G3BP2

Plasmid

#127105

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

G3BP2 (G3BP2 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pME-Cas9

Plasmid

#63154

Purpose

Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS

Depositor

Leonard Zon

Article

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Insert

Cas9

Use

CRISPR; Tol2 middle entry vector for gateway

Tags

Expression

Mutation

Promoter

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST-FLAG

Plasmid

#183511

Purpose

Gateway converted, destination vector with N-terminal Flag tag. For mammalian expression.

Depositor

Emanuele Panza

Article

Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

Type

Empty backbone

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2-U6:gRNA

Plasmid

#63157

Purpose

Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

Depositor

Leonard Zon

Article

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Type

Empty backbone

Use

CRISPR; Tol2 destination vector for gateway

Tags

Expression

Mutation

Promoter

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge