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Plasmid#189002Purposelipophagy-inducing adaptor protein, AAVDepositorInsertLDTS-eLIR, mCherry
UseAAVTagsHAExpressionMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGE013
Plasmid#108681PurposeAll-in-one genome editing vector in M. polymorpha (hygromycin resistant)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTNT-B18R-6His
Plasmid#58979Purposeexpresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNsDepositorInsertB18R
UseTagsHisExpressionBacterialMutationPromoterT7/Sp6Available sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSFV-Helper1
Plasmid#92073PurposeThis is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.DepositorInsertcDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
UseHelper plasmid, for run-off transcription and rna…TagsExpressionMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKOR1-mcs
Plasmid#181755PurposeAllele replacement for Staphylococcus aureusDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
2HA-pDEST-N
Plasmid#118373PurposeGateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2HAExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-3xFLAG-WPRE
Plasmid#127862PurposepAAV plasmid expressing 3xFLAG under the hSyn promoterDepositorInsert3xFLAG
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29b-mVenus-dspB(E184Q)-6xHis
Plasmid#176576PurposePlasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tag and mVenusExpressionBacterialMutationE184QPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-AlphaLobe
Plasmid#113255PurposeE. coli expression vector for His-MBP-Cas9 Alpha-Helical LobeDepositorInsertS. pyogenes Cas9 alpha-helical lobe
UseTags10xHis-MBPExpressionBacterialMutationresidues 56–714; See paper for descriptionPromoterT7Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
2HA-pDEST-C
Plasmid#118374PurposeGateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2HAExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
eGFP-CBP
Plasmid#169211PurposeExpression of eGFP-CBP protein for chitin visualizationDepositorInsertChitinase A1 Chitin Binding Domain
UseTags6xHis, Mxe Gyr Intein, and eGFPExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Red cGull
Plasmid#191853PurposeExpresses biosensor for cGMP in mammalian cellsDepositorInsertRed cGull
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
CD4-MKII-f-hLOV1-TEVcs(ENLYFQ/M)- Gal4
Plasmid#163027PurposeFLiCRE TF with fast kinetics for HEK293T expressionDepositorInsertCD4-MKII-f-hLOV1-TEVcs-Gal4
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-eGFP
Plasmid#157733Purposemammalian expression of untagged eGFP under control of a tetracycline-inducible promoterDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-R-GECO1.2
Plasmid#45494PurposeRed intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2DepositorInsertR-GECO1.2
UseTagsExpressionMammalianMutationR-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…PromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
p12x601
Plasmid#157785PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backboneDepositorInsertpMD2.G(spei/spei)&12x601_tetO
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_30bpLinker
Plasmid#157789PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_30bp linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
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CD4-MKII-hLOV1-TEVcs(ENLYFQ/M)- Gal4
Plasmid#163026PurposeFLiCRE TF for HEK293T expressionDepositorInsertCD4-MKII-hLOV1-TEVcs-Gal4
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only