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Plasmid#132941PurposeMLV packaging plasmid for N-tropic MLV gag-pol expressionDepositorInsertN-tropic MLV gag-pol
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGLO-GFP-3UAG
Plasmid#82501PurposeExpresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153DepositorInsertGreen Fluorescence Protein with 3 UAG codons
UseTagsExpressionBacterialMutationAdded an UAG codon at the amino acid position 3, …PromoterAraBADAvailable sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_APEX_FLAG_Ct
Plasmid#194069Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsFLAG-APEX-V5ExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1273
Plasmid#142774PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertDPF2 (DPF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTF101.2
Plasmid#134771PurposeA binary plasmid containing nopaline synthase promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2849
Plasmid#74112PurposeEnhanced mutant version of membrane targeted PH-miniSOG (Q103L) entry clone for more efficient ablation of cells when expressed under desired promoter.DepositorInsertminiSOG(Q103L)
UseGateway topo cloningTagsPHExpressionMutationPromoterAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTetO-ISce1-TetO
Plasmid#58506PurposeISce1 restriction site is flanked by Tet operator repeats, 96 on each sideDepositorInsertTet repeats flanking ISce1 restriction site
UseTagsExpressionBacterialMutationPromoterno promoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCV-Cas9
Plasmid#123643PurposeExpresses Cas9 with an N-terminal HUH-tag called PCV2DepositorInsertsUseTags(EAAAK)8 linker and His6 + SUMOExpressionBacterialMutation2-116PromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
P2RY2-Tango
Plasmid#66474PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertP2RY2 (P2RY2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF2678
Plasmid#144065PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPEG3 (PEG3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-Sharpin
Plasmid#195092PurposeGene Overexpression of Human SHARPINDepositorInsertSHARPIN (SHARPIN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LbABE8e(TadA-8e V106W)
Plasmid#138508PurposeA-to-G base editorDepositorInsertecTadA(8e V106W)-dLbCas12a
UseTagsExpressionMammalianMutationTadA-8e V106WPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGlnA-Ypet
Plasmid#98278Purposemammalian expression of GlnA-YpetDepositorInsertGlnA
UseTagsYpetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD1 (OTU+UIM, aa 287-481)
Plasmid#61406PurposeExpresses human OTUD1 (OTU+UIM domains) in E. coli.DepositorInsertOTUD1 (OTUD1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-286.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMBEC2
Plasmid#187596PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTn7CdrA-gfp(ASV)C
Plasmid#111617PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.DepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Multiplex-CRISPR-miR-200FAM-T2A-GFP
Plasmid#179856Purposeto delete miR200 family genes including miR-141, miR-200c, miR-429 and miR-200bDepositorInsertUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas12a-ABE8e-V106W
Plasmid#193646PurposeExpression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-TadA*8e-V106W, EGFP
UseTagsExpressionMammalianMutationTadA*8e-V106WPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-UBC-LUC-EF1 Hygro
Plasmid#129436Purposeexpress LUCDepositorInsertLUC
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.RGS4.C-HA
Plasmid#154847PurposeExpresses murine RGS4 protein with HA-tag at C-terminusDepositorInsertRegulator of G protein Signaling 4 (Rgs4 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only