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ArpC3-FLAG_pLib

Plasmid

#173680

Purpose

ArpC3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC3 (ARPC3 Human)

Use

Tags

TEV cleavage site and FLAG tag

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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ET1-human LRRC33

Plasmid

#111603

Purpose

Express human LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 (NRROS Human)

Use

Tags

PreScission/TEV/ProteinC/6xHIS/SBP/

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJW1886

Plasmid

#154333

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I^SEC (Lox511I)^TEV::degron::3xFLAG

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-DAXX-D/E-His

Plasmid

#179619

Purpose

Express DAXX D/E region in bacteria

Depositor

Xiaolu Yang

Article

Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

Insert

death domain associated protein (DAXX Human)

Use

Tags

GST, His, and TEV

Expression

Bacterial

Mutation

amino acids 414-505 of DAXX

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pJW1759

Plasmid

#154315

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::TEV::AID*::3xFLAG for NT slot

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry KNL1 wt hardened and RVSF->AAAA

Plasmid

#45226

Depositor

Iain Cheeseman, Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

mCherry KNL1 with RVSF-AAAA mutation, resistant to KNL1 siRNA (KNL1 Human)

Use

Tags

TEV S15

Expression

Mutation

RVSF 58 AAAA

Promoter

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only
pEJS663-pMCSG7-AcrIIC4Hpa

Plasmid

#113440

Purpose

Expresses a 6X-His tagged type II-C anti-CRISPR protein from H. parainfluenzae in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR with N-terminal 6xHis and TEV site

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

lacI

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS664-pMCSG7-AcrIIC5Smu

Plasmid

#113441

Purpose

Expresses a 6X-His tagged type II-C anti-CRISPR protein from S. muelleri in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR with N-terminal 6xHis and TEV site

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

lacI

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pJW1788

Plasmid

#154317

Purpose

CT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

linker::TEV::BioTag::AID*::3xFLAG donor for CT slot in the SapTrap cloning system

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1787

Plasmid

#154316

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

linker::TEV::BioTag::AID*::3xFLAG donor for NT slot in the SapTrap cloning system

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

Plasmid

#101208

Purpose

Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S355C, S867C, N497A, R661A and Q695A

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pbIG-Xph20-ETWV

Plasmid

#133034

Purpose

Expresses Xph20-ETWV in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph20-ETWV

Use

Tags

AviTag, His10, and TEV protease cleavage site

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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GST-ENLYFQC-PNT(46-138)-His6

Plasmid

#42919

Depositor

Barbara Imperiali

Article

Lukovic et al Angew Chem Int Ed Engl. 2009;48(37):6828

Insert

Ets-1 PNT domain (46-138) (Ets1 Mouse)

Use

Tags

6xHis, GST, and TEV protease cleavage site

Expression

Bacterial

Mutation

Contains the PNT domain (Serine 46 through Lysine…

Promoter

Ptac

Available since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

Plasmid

#101232

Purpose

Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

G528A, V583A, E584A, D585A, N588A and Q926A

Promoter

T7

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

Plasmid

#101229

Purpose

Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S355C, S867C, N692A, M694A, Q695A an…

Promoter

T7

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET-22HT-MSI1(7-192)R53E/R59E/R61E

Plasmid

#60355

Purpose

R53E R59E R61E triple mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R53E, R59E and R61E mutations; contains amino aci…

Promoter

T7

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pMCSG49-AlleyCat7

Plasmid

#42591

Depositor

Ivan Korendovych

Article

Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

Insert

mutated C-terminal portion of calmodulin gene (M76-K148)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

changed Phenylalanine 92 to Glutamine acid, Isole…

Promoter

T7

Available since

March 21, 2013

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-His-EWSR1-359-549

Plasmid

#195868

Purpose

Expresses the RRM-RG2-ZnF (359-549) of EWS

Depositor

David Libich

Article

Selig et al Biopolymers. 2023 May;114(5):e23536. doi

Insert

EWS 359-549 (EWSR1 Human)

Use

Tags

His8-linker-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBacI-KDAC6

Plasmid

#224346

Purpose

Expresses human KDAC6 (HDAC6) in insect cells

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC6 (HDAC6 Human)

Use

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFB-D3-ASK1

Plasmid

#226619

Purpose

Co-expression of D3 and ASK1 complex in insect cells

Depositor

Ning Zheng

Article

Shabek et al Nature. 2018 Nov;563(7733):652-656. doi:

Inserts

D3
ASK1 (SKP1 Mustard Weed)

Use

Tags

His tag followed with three copies of Msyb

Expression

Insect

Mutation

The flexible loop in D3 (Q478-D515) can be remove…

Promoter

Polyhedrin

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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