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Plasmid#67695Purposemiddle entry plasmid with dimerizable extracellular domain fused to Danio Flt4 cytoplasmic domainDepositorInsertFv2E-flt4
UseTagsExpressionMutationno stop codon; D1302E (see depositor's comme…PromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBALU3
Plasmid#69318PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of cfpDepositorInsertCFPint-FgF
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Kohinoor-Fibrilarin/pcDNA3
Plasmid#67781PurposeFibrilarin-targetted Kohinoor for mammalian cell expressionDepositorInsertKohinoor-Fibrilarin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBALU2
Plasmid#69317PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of yfpDepositorInsertYFPint-FgF
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP34
Plasmid#65501PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP35
Plasmid#65502PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP36
Plasmid#65504PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-Cer-cMycExpressionMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP42
Plasmid#65617PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSABAD14-111
Plasmid#61824PurposeNpuDnaB(delta283)dN11 inteinDepositorInsertNpuDnaB(delta283)dN11 intein
UseTagsGB1-H6ExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFUS2_a3a
Plasmid#50683PurposeIntermediate array vectorDepositorInsertLacZ + BsaI restriction sites
UseTALENTagsExpressionMutationPromoterAvailable sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFUS2_a3b
Plasmid#50684PurposeIntermediate array vectorDepositorInsertLacZ + BsaI restriction sites
UseTALENTagsExpressionMutationPromoterAvailable sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFUS2_a4a
Plasmid#50685PurposeIntermediate array vectorDepositorInsertLacZ + BsaI restriction sites
UseTALENTagsExpressionMutationPromoterAvailable sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFUS2_a4b
Plasmid#50686PurposeIntermediate array vectorDepositorInsertLacZ + BsaI restriction sites
UseTALENTagsExpressionMutationPromoterAvailable sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
Phamret-H2B/pcDNA3
Plasmid#51955Purposemammalian expression of Phamret, a monomeric photoconvertible fluorescent protein, fused to H2BDepositorInsertPhamret
UseTagshuman H2BExpressionMammalianMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Phamret-Fibrillarin/pcDNA3
Plasmid#51954Purposemammalian expression of Phamret, a monomeric photoconvertible fluorescent protein, fused to Fibrillarin for nucleolar localizationDepositorInsertPhamret
UseTagsFibrillarinExpressionMammalianMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Xl-VSP2 GFP
Plasmid#51886Purposeexpression of Xl-VSP2-GFP fusion in Xenopus oocytes or mammalian cellsDepositorInsertVSP2
UseXenopus oocyte expressionTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFUS2_b3
Plasmid#50697PurposeIntermediate array vectorDepositorInsertLacZ + BsaI restriction sites
UseTALENTagsExpressionMutationPromoterAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFUS2_b2
Plasmid#50696PurposeIntermediate array vectorDepositorInsertLacZ + BsaI restriction sites
UseTALENTagsExpressionMutationPromoterAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSABAD518
Plasmid#46374DepositorInsertTvoVMA intein, inactive
UseTagsH6-GB1 and SH3ExpressionBacterialMutationN186A, T+1A mutations, inactivePromoterpBADAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Tcrb-V13 in situ probe
Plasmid#45622DepositorInsertTcrb-V13 in situ probe (Tcrb Mouse)
UseIn situTagsExpressionMutationfragment contains bp# 533-1058 of X67128PromoterAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only