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Plasmid#65329PurposeCEN plasmid as receiving vector for exogenous pathwayDepositorInsertURR1-RFP-Leu2-URR2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMV-URR2
Plasmid#65332Purposestandard plasmids for exogenus pathway assembly haboring recombination sequence 2 (Universal Recombination Region 2) which could be released and ligated to series of TUsDepositorInsertURR2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMV-URR1
Plasmid#65331Purposestandard plasmids for exogenus pathway assembly haboring recombination sequence 1(Universal Recombination Region 1) which could be released and ligated to series of TUsDepositorInsertURR1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pN1-mCherry-NKD2
Plasmid#176641PurposeExpresses NKD2-mCherry fusion protein in mammalian cellsDepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_rSERT_C109A_Y107C
Plasmid#190174PurposeFor expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathwayDepositorInsertSLC6A4 Serotonin Transporter (Slc6a4 Rat)
UseTagsFLAG, His6, and c-mycExpressionMammalianMutationchanged Cys109 to alanine, changed tyr107 to cyst…PromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1mcherry-NKD2S31A
Plasmid#174332Purposeencodes human NKD2 S31A mutant cDNA with a C-terminal mCherry fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationchanged Serine 31 to AlaninePromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-NKD2S31A
Plasmid#174331Purposeencodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsMycHisExpressionMammalianMutationchanged Serine 31 to AlaninePromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC030.306
Plasmid#171151PurposeMevalonate production pathway with J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-mvaES
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J3-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
FoxFTVC+bpFOG_MEK-S216D, S220E
Plasmid#107522PurposeConstitutive activation of FGF/MAPK pathway by MEK-S216D, S220E mutantDepositorInsertMEK
UseTagsExpressionMutationchanged Serine 216 to Aspartic Acid and Serine 22…PromoterTVC-specific FoxF enhancer (FoxFTVC+bpFOG)Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMV-TRP1
Plasmid#65335Purposestandard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUsDepositorInsertTRP1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 EE Jip1b
Plasmid#52127Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)
UseTagsEEExpressionMammalianMutationPromoterAvailable sinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Pact1-ITE-Tadh1
Plasmid#127723PurposeYeast pathway position 1. ITE transcription unit with the ACT1 promoter and ADH1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPact1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Ppre3-ITE-Teno1
Plasmid#127724PurposeYeast pathway position 1. ITE transcription unit with the PRE3 promoter and ENO1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPpre3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Pact1-ITE-Tdpp1
Plasmid#127725PurposeYeast pathway position 1. ITE transcription unit with the ACT1 promoter and DPP1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPact1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Phta1-ITE-Tvma2
Plasmid#127726PurposeYeast pathway position 1. ITE transcription unit with the HTA1 promoter and VMA2 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPhta1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Prps3-CAD-Tcyc1
Plasmid#127728PurposeYeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPrps3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Peft2-CAD-Tklura3
Plasmid#127729PurposeYeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPeft2Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Psmtef1-CAD-Tyhi9
Plasmid#127730PurposeYeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsmtef1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Pfba1p-ACO-Tgpm1
Plasmid#127731PurposeYeast pathway position 4. ACO transcription unit with the FBA1 promoter and GPM1 terminator.DepositorInsertaconitase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfba1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Pykt6-ACO-Tprm9
Plasmid#127732PurposeYeast pathway position 4. ACO transcription unit with the YKT6 promoter and PRM9 terminator.DepositorInsertaconitase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPykt6Available sinceAvailabilityAcademic Institutions and Nonprofits only