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pEM7070

Plasmid

#214008

Purpose

PrpsM driven expression of ssrA-tagged mNeonGreen (ASV)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mNeonGreen-ASV

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM7074

Plasmid

#214012

Purpose

PrpsM driven expression of ssrA-tagged mCerulean (ASV)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mCerulean-ASV

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM7068

Plasmid

#214006

Purpose

PrpsM driven expression of ssrA-tagged eGFP (ASV)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

eGFP-ASV

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET28CLY7

Plasmid

#21766

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 7 SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
iDuet011a

Plasmid

#17628

Depositor

Linzhao Cheng

Article

Zhou et al Stem Cells. 2007 Mar . 25(3):779-89.

Insert

TetO7 - UBC promoter - GFP - PGK promoter - Hygro

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pSLQ11474

Plasmid

#183624

Purpose

PB-STAT6RE-minCMV-BFP-Triplex-crAAVS1_6-SV40pA

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

Cas12 Guide (crAAVS1_6)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAd5-B3/4

Plasmid

#175785

Purpose

A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B3 and pAd5-B4.

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Adenovirus 5 genomic region 10862-18018

Use

Adenoviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV1

Plasmid

#78633

Purpose

GESTALT V1 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT target V1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD_A5801

Plasmid

#127223

Purpose

35S::Luciferase

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_A5803

Plasmid

#127224

Purpose

CmYLCV::Luciferase

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO5.01

Plasmid

#121418

Purpose

pUC19_BsaI-dCas9-BsaI

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

dcas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTXB1-AbaSFI

Plasmid

#86900

Purpose

Express CBD and intein tag containing AbaSI enzyme full length

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

AbaSDFI

Use

Tags

CBD

Expression

Mutation

Promoter

T7

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMyBADNT

Plasmid

#84690

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

AraC

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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PaBphP 1-497

Plasmid

#36920

Depositor

Keith Moffat

Article

Yang et al Nature. 2011 Oct 16;479(7373):428-32. do

Insert

PaBphP-PCD

Use

Tags

His

Expression

Bacterial

Mutation

Contains the photosensory core domain coding regi…

Promoter

T7

Available since

May 18, 2012

Availability

Academic Institutions and Nonprofits only
pJK59-ATG17-GFP

Plasmid

#44175

Depositor

James Hurley

Article

Ragusa et al Cell. 2012 Dec 21;151(7):1501-12. doi: 1

Insert

Atg17 (ATG17 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

none

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
p11U1ADb

Plasmid

#27384

Depositor

David Bartel

Article

Shechner et al Science. 2009 Nov 27. 326(5957):1271-5.

Insert

U1A A1-98 Y31H/Q36R (SNRPA Human)

Use

Tags

Expression

Bacterial

Mutation

A1-98 Y31H/Q36R double mutant

Promoter

Available since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only
pET28CLY6

Plasmid

#21765

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 6 SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
peZinCh-NB

Plasmid

#24173

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eZinCh-NB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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pTHSSe_44

Plasmid

#109242

Purpose

pSC101 var2 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSR1048

Plasmid

#208738

Purpose

Characterization plasmid for PRelA (sfgfp expressed from PRelA on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PRelA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PRelA

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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