Addgene

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pNK110d

Plasmid

#156321

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Tags

Expression

Mutation

WT antiRBS engineered to orthogonal antiRBS, PS e…

Promoter

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4164342

Plasmid

#153553

Purpose

Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

accatg Kozak-START linker

Expression

Mammalian

Mutation

Gordon et al recoded

Promoter

Available since

June 16, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4164341

Plasmid

#153552

Purpose

Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

accatg Kozak-START linker

Expression

Mammalian

Mutation

Gordon et al recoded

Promoter

Available since

June 16, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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LPhP-pHG165

Plasmid

#90047

Purpose

Constitutive expression of a chimeric LacI:PurR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Tungtur et al J Mol Biol. 2010 Jan 29;395(4):785-802.

Insert

LPhP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pICSL80012

Plasmid

#50331

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, YFP variant of GFP (A. victoria)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL00002

Plasmid

#50347

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Linker for joining modules with 3' TTCG overhangs to modules with 5' GTGG overhangs

Use

Plant expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL00001

Plasmid

#50346

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Linker for joining modules with 3' GTGG overhangs to modules with 5' TTCG overhangs

Use

Plant expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL50012

Plasmid

#50311

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal V5 tag (Simian Virus 5)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL50013

Plasmid

#50312

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal T7 tag (bacteriophage T7 gene 10)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH44233

Plasmid

#50288

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR CMV2 (Cucumber Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH44179

Plasmid

#50290

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5' UTR, RbcS2B (AT5g38420, A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH37431

Plasmid

#50306

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

signal peptide, RAmy3A (O. sativa)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH37326

Plasmid

#50307

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

signal peptide, NbCRT calreticulin (Nicotiana benthamiana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH45089

Plasmid

#50254

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (double), 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH42760

Plasmid

#50257

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, spm (Z. mays)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH45180

Plasmid

#50260

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, RbcS3B (AT5g38410, A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH45131

Plasmid

#50262

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, LHB1B2 (AT2g34420, A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-c3HA-spo5DSR

Plasmid

#49872

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Expression

Mutation

Promoter

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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PL06010B1E06 (smedwi3)

Plasmid

#35697

Depositor

Phillip Newmark

Article

Zayas et al Proc Natl Acad Sci U S A. 2005 Dec 20;10

Insert

Smedwi3

Use

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pJB008

Plasmid

#22647

Depositor

Lynn Hudson

Article

Hudson et al Proc Natl Acad Sci U S A. 1989 Oct . 86(

Insert

Human genomic PLP (PLP1 Human)

Use

Phagemid

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only