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Plasmid#214880PurposeLentiviral vector encoding RfxCas13d targeting CD46 guideDepositorInserthU6-crCD46-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCH49 (crRNA expression vector)
Plasmid#217338PurposeCROP-seq crRNA expression vector with GFP and neo markerDepositorInsertAsDR-filler-AsDR8, GFP-neo
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV TRE-GFP-TMD-DLL1
Plasmid#194936PurposeLV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.DepositorInserteGFP-(PDGFR)TMD-rDLL1 ICD (WT)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-XI-Neo-GST- AMPK- Kinase Dead (K to R)
Plasmid#139844PurposeLentiviral expression of GST-tagged rat AMPK- Kinase Dead (K to R)DepositorInsertAMPK-Kinase-dead (Prkaa2 Rat)
UseLentiviralTagsGSTExpressionMutationK47RPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry2(11)_TagBFP
Plasmid#82606PurposeExpresses sfCherry2(11) and TagBFP fusion in mammalian cellsDepositorInsertsfCherry2(11)_TagBFP
UseLentiviralTagsExpressionMammalianMutationchanged Gly12 to Ala in sfCherry11+PromoterSFFVAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-GFP-V5_IDG-K
Plasmid#135242PurposeGateway destination clone of GFP (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertGFP-V5
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-2XboxB
Plasmid#75391PurposesgRNA1-2XboxBDepositorInsertsgRNA1-2XboxB
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgCXCR4-2
Plasmid#46917PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CXCR4 geneDepositorInsertsUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMV and U6Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYT1 reporter
Plasmid#197275PurposeEncodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYT1 and HaloTag (Syt1 Rat)
UseLentiviralTagsFLAGExpressionMutationN/APromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a
Plasmid#177435PurposeTo express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.DepositorInsertPhosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
dimericTomato-1xwGBD
Plasmid#176099PurposeVisualization of active Cdc42DepositorInsertWAS (WAS Human)
UseLentiviralTagsExpressionMutationPromoterCMVdelAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRL-5xTetO-pEF-H2B-Citrine-SV40
Plasmid#186966PurposeLentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.DepositorInsert5xTetO-pEF-H2B-Citrine-SV40
UseLentiviralTagsExpressionMammalianMutationPromoter5xTetO-pEF-1alphaAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF2116
Plasmid#144385PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNCOA6 (NCOA6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL003-ishRNA-beta-catenin-2
Plasmid#36299DepositorInsertshRNA of beta-catenin-2 (CTNNB1 Human)
UseLentiviral and RNAiTagsIRES-RFP-PuroExpressionMammalianMutationPromoterTetO-H1Available sinceAug. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLV-mTurquoise2-Caspase-1-mNeonGreen
Plasmid#166784PurposeFRET analysis (pro caspase-1 cleavage) in living cellsDepositorInsertmTurquoise2-caspase-1-mNeonGreen (CASP1 Human)
UseLentiviralTagsmNeonGreen and mTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGH045_EGFP
Plasmid#85411Purposelentiviral expression vector for GFPDepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTiger
Plasmid#1728PurposeThis is a 3rd generation lentiviral transfer vector (highest titer) from Nolan lab's FELIX vector systemDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Hygro
Plasmid#167907PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER-21-RUNX1
Plasmid#97043PurposeExpresses RUNX1 in mammalian cellsDepositorInsertRunt Related Transcription Factor 1 (RUNX1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTet onAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-FASCIN
Plasmid#31207DepositorInsertFASCIN (FSCN1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only