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pDDGFP-Leu2d-GgKDELR2 H12A

Plasmid

#123619

Purpose

Expresses GgKDELR2 with His12Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

His12Ala

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 R47A

Plasmid

#123620

Purpose

Expresses GgKDELR2 with Arg47Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg47Ala

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 R47K

Plasmid

#123621

Purpose

Expresses GgKDELR2 with Arg47Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg47Lys

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDDGFP-Leu2d-GgKDELR2 E127A

Plasmid

#123622

Purpose

Expresses GgKDELR2 with Glu127Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Glu127Ala

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 E127Q

Plasmid

#123623

Purpose

Expresses GgKDELR2 with Glu127Gln mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Glu127Gln

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDDGFP-Leu2d-GgKDELR2 Y158F

Plasmid

#123624

Purpose

Expresses GgKDELR2 with Tyr158Phe mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Tyr158Phe

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDDGFP-Leu2d-GgKDELR2 R159A

Plasmid

#123625

Purpose

Expresses GgKDELR2 with Arg159Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg159Ala

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLAP-BUBR1-3A

Plasmid

#114054

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

BUBR1-3A (BUB1B Human)

Use

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-S670A…

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLAP-BUBR1-3D

Plasmid

#114055

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

BUBR1-3D (BUB1B Human)

Use

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-S670D…

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLAP-MIS12-KARD-3D

Plasmid

#114058

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD-3D (BUB1B Human)

Use

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

BUBR1-S670D, S676D and T680D

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLAP-MIS12-KARD-3A

Plasmid

#114059

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD-3A (BUB1B Human)

Use

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

BUBR1-S670A, S676A and T680A

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

Plasmid

#101207

Purpose

Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N497A/R661A/Q695A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N497A, R661A and Q695A

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

Plasmid

#101201

Purpose

Bacterial expression plasmid for SpCas9, REC3 FRET variant

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S701C/S960C

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S701C and S960C

Promoter

T7

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

Plasmid

#101214

Purpose

Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S355C/S867C/K855A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S355C, S867C and K855A

Promoter

T7

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

Plasmid

#101200

Purpose

Bacterial expression plasmid for SpCas9, REC2 FRET variant

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/E60C/D273C

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, E60C and D273C

Promoter

T7

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-22HT-MSI1(7-192)Q30D

Plasmid

#60346

Purpose

Q30D mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

Q30D; contains amino acids 7-192 of Msi1

Promoter

T7

Available since

Dec. 1, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)R53E

Plasmid

#60352

Purpose

R53E mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R53E; contains amino acids 7-192 of Msi1

Promoter

T7

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)G64A

Plasmid

#60351

Purpose

G64A mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

G64A; contains amino acids 7-192 of Msi1

Promoter

T7

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)H83L

Plasmid

#60350

Purpose

H83L mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

H83L; contains amino acids 7-192 of Msi1

Promoter

T7

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)G35D

Plasmid

#60347

Purpose

G35D mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

G35D; contains amino acids 7-192 of Msi1

Promoter

T7

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only