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Plasmid#162777PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2_ZIKV C
Plasmid#145359PurposeCell-free/mammalian expression vector for Zika virus capsid protein with N-term mCherryDepositorInsertZIKV Capsid
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2_ZIKV PrM
Plasmid#145360PurposeCell-free/mammalian expression vector for Zika virus PrM protein with N-term mCherryDepositorInsertZIKV PrM
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceMarch 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2_ZIKV NS4B
Plasmid#145367PurposeCell-free/mammalian expression vector for Zika virus NS4Bprotein with N-term mCherryDepositorInsertZIKV NS4B
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
DCIP
Plasmid#193860PurposeEncodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.DepositorInsertNLS-mCherry-GFP1-10
UseTagsSV40 NLSExpressionPlantMutationPromoter35SAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN-R2-mCsfGFP-L3
Plasmid#178602PurposeIt can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.DepositorInsertTandem fluorescent protein timers (tFTs)
UseEntry vector for gateway cloningTagsmCherry superfolder GFPExpressionMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
S12.2_Bridge + Target
Plasmid#127671PurposeAmpicillin resistant multiplexing backbone with CRY2HA and mCherryDepositorTypeEmpty backboneUseCRISPRTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-1xTS
Plasmid#162778PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2_ZIKV NS4A
Plasmid#145366PurposeCell-free/mammalian expression vector for Zika virus NS4A protein with N-term mCherryDepositorInsertZIKV NS4A
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-H2A-Akt-STOPS
Plasmid#175010PurposeAkt-STOPS targeted to the nucleus via histone H2A.DepositorInsertH2A-mCherry-Akt-STOPS
UseTagsHistone H2A and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
sgFFL
Plasmid#59877Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene.DepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-C
Plasmid#183160PurposePlant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of mCherry.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2_ZIKV NS2A
Plasmid#145363PurposeCell-free/mammalian expression vector for Zika virus NS2A protein with N-term mCherryDepositorInsertZIKV NS2A
UseTagsHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK2067
Plasmid#171845PurposeC. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)DepositorInserthis-58, eGFP, mCherry
UseTagsN-terminal Tag OLLAS, V5 and C-terminal Tag PEST…ExpressionWormMutationboxB wild type and hairpin mutantPromotermex-5 promoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSR06
Plasmid#69152Purposeread-outloxN mCherry to GFP switch for integration on ttTi5605, Mos Chr IIDepositorInsertsmCherry
eGFP
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0 and codon-optimzed index…Promoterrps-27Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 1277
Plasmid#53017PurposeAmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) mCherry::Asp terminator + ColE1DepositorInsertsGFP
mCherry
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree _KA2_ZIKV NS5
Plasmid#145368PurposeCell-free/mammalian expression vector for Zika virus NS5 protein with N-term mCherryDepositorInsertZIKV NS5
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHL 1278
Plasmid#53018PurposeAmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS (st7) gfp::Asp terminator + ColE1DepositorInsertsmCherry
GFP
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJZC35
Plasmid#62325PurposesgRNA for mammalian cells with mCherry markerDepositorInsertsgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJZC102
Plasmid#62337PurposesgRNA for mammalian cells with mCherry markerDepositorInsertsgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only