Addgene

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FKBP-GFP-Sec61β

Plasmid

#172442

Purpose

Expression of ER protein Sec61beta with an N-terminal FKBP-GFP tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Protein transport protein Sec61 subunit beta

Use

Tags

FKBP-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-pHluorin

Plasmid

#185537

Purpose

Expresses LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

Promoter

CamKII

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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612 p3E-2A-mCherryCAAXpA

Plasmid

#195971

Purpose

Gateway p3E 2A fluorophore

Depositor

Christian Mosimann, Kristen Kwan

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-mCherry-CAAX (membrane)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-DsRed2

Plasmid

#15777

Depositor

Edward Callaway

Article

Wickersham et al Neuron. 2007 Mar 1. 53(5):639-47.

Insert

DsRed2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual wt p110α/p85α

Plasmid

#199302

Purpose

co-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins

Depositor

Peter Vogt

Article

Favalli et al Nat Chem. 2021 Jul;13(7):714. doi: 10.10

Insert

His-tagged p110α wt and Avi-tagged p85α wt (PIK3CA, PIK3R1 Human)

Use

Tags

Avi and His

Expression

Insect

Mutation

Promoter

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-iRFP670-CaaX

Plasmid

#170464

Purpose

Expresses iRFP670 fluorophore targeted to the plasma membrane using a prenylation motif

Depositor

John James

Article

Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

Insert

iRFP670-CaaX

Use

Lentiviral

Tags

CaaX

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pWPI-IRES-Bla-Ak-ACE2-TMPRSS2

Plasmid

#154983

Purpose

Lentiviral vector expressing Human ACE2 and TMPRSS2

Depositor

Sonja Best

Insert

ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-cMYC promoter

Plasmid

#114724

Purpose

reporter luciferase construct

Depositor

Martine Roussel

Article

Roussel et al Oncogene. 1994 Feb;9(2):405-15.

Insert

C-Myc promoter region

Use

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK1

Plasmid

#53159

Purpose

Expresses GST-HA tagged human MEK1 from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry-NLS

Plasmid

#39319

Depositor

Martin Offterdinger

Article

Micutkova et al Int J Cancer. 2012 Apr 1;130(7):1544-57.

Insert

mCherry-3xNLS

Use

Tags

3xSV40LT NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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