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His-ATG3-Y210A

Plasmid

#202780

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y210A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Use

Tags

6xHis, TEV

Expression

Bacterial

Mutation

Y210A

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-K208D

Plasmid

#202785

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K208D-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Use

Tags

6xHis, TEV

Expression

Bacterial

Mutation

K208D

Promoter

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-C264A

Plasmid

#202778

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-C264A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Use

Tags

6xHis, TEV

Expression

Bacterial

Mutation

C264A

Promoter

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-R265A

Plasmid

#202783

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-R265A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Use

Tags

6xHis, TEV

Expression

Bacterial

Mutation

R265A

Promoter

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-H266A

Plasmid

#202784

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H266A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Use

Tags

6xHis, TEV

Expression

Bacterial

Mutation

H266A

Promoter

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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hTln1-R1R2

Plasmid

#191440

Purpose

Expresses the human TLN1 R1R2 domains in bacteria

Depositor

Ben Goult

Article

Gallego-Paez et al J Cell Biol. 2023 May 1;222(5):e20220901

Insert

Human Talin1 R1R2 domains (TLN1 Human)

Use

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W5F

Plasmid

#198443

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W5F

Promoter

T7 promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W89F

Plasmid

#198447

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W89F

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W83F

Plasmid

#198446

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W83F

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W69F

Plasmid

#198445

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W69F

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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eZiPro

Plasmid

#198434

Purpose

Activity/inhibition assays, 19F-NMR

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W50F

Plasmid

#198444

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W50F

Promoter

T7 promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(T304I)

Plasmid

#177133

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(K72A)

Plasmid

#177132

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Tac

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_162 SpCas9 KES(1107-1109)GG

Plasmid

#179526

Purpose

For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 KES(1107-1109)GG

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

replaced KES (residues 1107-1109) with GG

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_163 SpCas9 K233A/K234A/K253A/K263A

Plasmid

#179527

Purpose

For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 K233A/K234A/K253A/K263A

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed lysines 233, 234, 253, and 263 to alanine

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(K206A/K244A)

Plasmid

#177135

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A) with a TEV protease site located between the GST tag and PolB(K206A/K244A)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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EcRfaHKOW-L1L2NusG

Plasmid

#113126

Purpose

Expresses C-terminus of E. coli RfaH with L1 and L2 loops replaced with NusG residues

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

RfaH (rfaH Escherichia coli)

Use

Tags

His6-MBP-N10-TEV

Expression

Bacterial

Mutation

Deleted residues 1-107, replaced L1 and L2 loops …

Promoter

T7

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(214-535)

Plasmid

#135439

Purpose

Expresses SFPQ 214-535 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2 R159K

Plasmid

#123626

Purpose

Expresses GgKDELR2 with Arg159Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Use

Tags

TEV-GFP-His

Expression

Yeast

Mutation

Arg159Lys

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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