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Plasmid#101234PurposeBacterial expression plasmid for SpCas9 Cluster 3 + Q926A variantDepositorInsertSpCas9 variant T657A/G658A/W659A/R661A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationT657A, G658A, W659A, R661A and Q926APromoterT7Available sinceNov. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant
Plasmid#101220PurposeBacterial expression plasmid for SpCas9 Cluster 1 + Q926A variantDepositorInsertSpCas9 variant N692A/M694A/Q695A/H698A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationN692A, M694A, Q695A, H698A and Q926APromoterT7Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT-CuSeCat EE
Plasmid#42569DepositorInsertmutated calmodulin gene
UseTagsPolyhistidine (6×His) region, TEV recognition sit…ExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Valin…PromoterT7Available sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)
Plasmid#60245PurposeExpress the RBD of mouse MSI1 in bacterial cells for protein purificationDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationcontains aa7-192 of Msi1PromoterT7Available sinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)R61E
Plasmid#60353PurposeR61E mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationR61E; contains amino acids 7-192 of Msi1PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJExpress401-KDAC4 CD H976Y
Plasmid#224250PurposeExpresses human KDAC4 (HDAC4) catalytic domain H976Y in E. coliDepositorInsertKDAC4 (HDAC4 Human)
UseTagsTEV-cleavable His6ExpressionBacterialMutationOnly includes residues 648-1083 (catalytic domain…PromoterT5Available sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV_TRE3G_hRTN3E-Halo
Plasmid#216252PurposeTet-inducible hRTN3E (long form) expression vector, expresses hRTN3E-TEV-Halo, Lentiviral vectorDepositorInsertreticulon-3 isoform e (RTN3 Human)
UseLentiviralTagsHaloTagExpressionMutationPromoterTRE3GAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis-WzzE
Plasmid#196661PurposeExpression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
UseTagsTEV-cleavable His6ExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGST-WzzE
Plasmid#196662PurposeExpression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
UseTagsTEV-cleavable GSTExpressionBacterialMutationValine insertion at position 2.PromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPglL-strep-mCherry
Plasmid#196664PurposeExpression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coliDepositorInsertPglL
UseTagsTEV-cleavable distrep tag II and mCherryExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPglL-strep-mClover3
Plasmid#196665PurposeExpression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coliDepositorInsertPglL
UseTagsTEV-cleavable di-strep tag II and mClover3ExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-GST-distrepII
Plasmid#196666PurposeExpression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsGST and TEV-cleavable di-strep tag IIExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-distrepII
Plasmid#196667PurposeExpression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsTEV-cleavable di-strep tag IIExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-strep-mClover3
Plasmid#196668PurposeExpression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsTEV-cleavable di-strep tag II and mClover3ExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_DeltaN24
Plasmid#214327PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationDeletion of first 24 amino acidsPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1-Cdc45
Plasmid#208796PurposeXenopus laevis FLAG5-His10-TEV-Cdc45DepositorInsertCdc45 (cdc45.L Frog)
UseTagsFLAG and HisExpressionBacterialMutationPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-K62D/K64D
Plasmid#202786PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K62D/K64D-stopDepositorInsertATG3 (ATG3 Human)
UseTags6xHis, TEVExpressionBacterialMutationK62D, K64DPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-H262A
Plasmid#202782PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H262A-stopDepositorInsertATG3 (ATG3 Human)
UseTags6xHis, TEVExpressionBacterialMutationH262APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-T244A
Plasmid#202781PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-T244A-stopDepositorInsertATG3 (ATG3 Human)
UseTags6xHis, TEVExpressionBacterialMutationT244APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-Y209A
Plasmid#202779PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y209A-stopDepositorInsertATG3 (ATG3 Human)
UseTags6xHis, TEVExpressionBacterialMutationY209APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only