Addgene

Showing: 4781 - 4800 of 5918 results

Results per page:

Sort results by:

pcDNA3-nuc-GsCT

Plasmid

#205517

Purpose

Nuclear-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

nuc-GsCT (GNAS Synthetic, Human)

Use

Tags

Histone H2A and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mCh-KIF1A*-strep

Plasmid

#120165

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 1A (Kif1a Mouse)

Use

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF1A: 1-405 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-iCre-2A-cherry

Plasmid

#182246

Purpose

Expresses iCre linked to mCherry. The promoter is a fragment of the hdc gene promoter which drives pan neuronal expression.

Depositor

William Wisden

Article

Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

Insert

pan neuronal gene promoter (hdc) fragment driving iCre recombinase and 2A linked to mCherry

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

fragment of histidine decarboxylase gene promoter…

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-ZIP(WT)

Plasmid

#27134

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
mCh-KIF17*-strep

Plasmid

#120167

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 17 (KIF17 Human)

Use

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF17: 1-509 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p28-2iDP-puro

Plasmid

#88893

Purpose

Dual-intron DMD platform plasmid. Co-expresses DMD platform segment, firefly luciferase, puroR and mCherry. Plasmid carries phiC31 and Bxb1 attB sites.

Depositor

Michele Calos

Article

Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

Inserts

Dual-intron DMD platform
luciferase
puromycin resistance enzyme
mCherry

Use

Tags

Expression

Mammalian

Mutation

Truncated version of the dystrophin protein (tran…

Promoter

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LAMP-mChF-AIP

Plasmid

#61524

Purpose

Expression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIP

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

lysosomal-associated membrane protein 1 (LAMP1 Human)

Use

Tags

AIP and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mCh-KIF13A*-strep

Plasmid

#120166

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF13A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 13A (KIF13A Human)

Use

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF13A: 1-639 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGMC00013

Plasmid

#172525

Purpose

sgRNA against mouse Mr1

Depositor

Raj Chari

Article

Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

Insert

Mr1 (Mr1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGMC00014

Plasmid

#172526

Purpose

non targeting sgRNA

Depositor

Raj Chari

Article

Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

Insert

Non-targeting guide

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mCh-KIF5A*-strep

Plasmid

#120163

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5A (Kif5a Mouse)

Use

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5A: 1-559 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-ZIP(RK)

Plasmid

#27136

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Arginines in the SH2 domains mutated to lysines (…

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo

Plasmid

#74919

Purpose

Mammalian expression of mKlf2 and mNanog-mCherry

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Inserts

Klf2 (Klf2 Mouse)
Nanog (Nanog Mouse)

Use

Tags

T2A and mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only
sgCRY2

Plasmid

#197457

Purpose

Expresses sgRNA(CRY2 gene) in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCRY2-1-hU6-sgCRY2-2 (Cry2 Mouse)

Use

AAV

Tags

mCherry (K14E, N210D, E249G)

Expression

Mutation

Promoter

U6, hSyn

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC8_MF-p10-mCh PH-mCh (VE5625)

Plasmid

#139769

Purpose

Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an mCherry cDNA under each promoter.

Depositor

Arnaud Poterszman

Article

Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

Insert

mCherry fluorescent protein

Use

Tags

Expression

Insect

Mutation

Promoter

PH or p10

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-ZIP(FF)

Plasmid

#27135

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Tyrosines in ITAMS 1-3 mutated to phenylalanines …

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pSN33

Plasmid

#100118

Purpose

expresses a chimeric KLF4-repressor fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCherry-P2A-KLF4 fused to full length HP1 alpha (KLF4, CBX5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-855

Plasmid

#218013

Purpose

mCherry fluorescent protein

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

mCherry

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

Plasmid

#217662

Purpose

expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA1_Cas9-hGem_Smc4

Plasmid

#217667

Purpose

expresses Cas9-hGem and guideRNA for tagging of SMC4

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA1 targeting SMC4 C-terminal (SMC4 Human)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge