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Plasmid#107582PurposeB. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic Biology; E. coli and bacillus shuttle v…TagsB. megaterium DSM319 xylA leader sequence (MTSSKI…ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterfba promoter Bacillus megaterium DSM319Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-PKI-GFP
Plasmid#118482PurposeGFP-tagged Protein Kinase A inhibitor peptide.DepositorInsertPKI-GFP
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Blast
Plasmid#207710PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Blast cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Blast-moxGFP
Plasmid#207677PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-moxGFP cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
VARP GFP-pLXIN
Plasmid#62950Purposefull length human VARP with C-terminal EGFP tag cloned into pLXINDepositorInsertVARP (ANKRD27 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationsilent mutations of amino acids 422-428 to produc…PromoterLTRAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tet-CA-Src-GFP
Plasmid#83469PurposeLentiviral vector for doxycycline-inducible expression of constitutively active c-Src in mammalian cellsDepositorInsertSrc (SRC Chicken)
UseLentiviralTagsEGFPExpressionMammalianMutationY527F (constitutively active)Promoterdoxycycline-inducible promoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
NET47 pEGFP-N2 (593)
Plasmid#61986Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET47 (TM7SF2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
acGFP WC visual barcode
Plasmid#158672Purposetagging clonal populations with whole cell acGFP fluorescent proteinDepositorInsertacGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-phoAp-GFPuv
Plasmid#139072PurposeLow phosphate inducible promoter phoAp for GFP expressionDepositorInsertphoAp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tg(mpeg1:EGFP-Mmu.Rpl10a)
Plasmid#128840PurposeRNA isolation from machrophages via TRAPDepositorInsertEGFP-L10a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
LBR-FKBP-GFP
Plasmid#222413PurposeTo express LBR with C-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertLBR (LBR Human)
UseTagsFKBP-GFPExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCVsin-PGK-GFP
Plasmid#35615DepositorInsertGFP
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVXPuro-mGFP-LIC2
Plasmid#66601PurposeExpresses Dynein-1 LIC2 as a mGFP fusionDepositorInsertLIC2 (DYNC1LI2 Human)
UseLentiviralTagsmGFPExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
NLS pEGFP-N3 (1299)
Plasmid#62043Purposemammalian expression of NLS-EGFPDepositorInsertSV40 NLS
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID*-GFP
Plasmid#99521PurposeC-terminal AID*-GFP degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(71-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTagsGFPExpressionMutationPromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMVS102_P3-GFP-NATMX6
Plasmid#99048PurposeEGFP reporter with six binding sites for the Zif268 DNA binding domainDepositorInsertsMcIsaac 2014 P3 promoter
eGFP
clonNAT resistance
UseTagsExpressionYeastMutationGAL1 promoter with 4 GAL4 sites removed and repla…PromoterMcIsaac 2014 P3 promoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-MYO7A-TAIL
Plasmid#89585PurposeExpression plasmid for a positive control bait protein in NanoSPD 2.0 assays.DepositorInsertMYO7A (Myo7a Mouse)
UseTagsEGFPExpressionMammalianMutationTruncation encoding amino acids 870-2166 of MYO7A…PromoterCMVAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-AP180-GFP
Plasmid#186577PurposeExpression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.DepositorInsertAP180 (Snap91 Rat)
UseTagsFKBP N-terminal GFP C-terminalExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-mGFP-TAX1BP1
Plasmid#192803PurposeStable expression of mGFP-TAX1BP1 in mammalian cellsDepositorInsertNCOA4 (NCOA4 Human)
UseTagsmonomeric EGFP (A206K)ExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFPLaminA-N195K
Plasmid#32708DepositorInsertLamin A (LMNA Human)
UseTagsGFPExpressionMammalianMutationN195KPromoterCMVAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only