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Plasmid#80769PurposeExpresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).DepositorInsertIFT88 gRNA#1 (IFT88 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα1(I)
Plasmid#119843PurposeExpresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 chain (Col1a1 Mouse)
UseTagseGFPExpressionMammalianMutationCol1a1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiSSTe10_C1V1_eYFP
Plasmid#213816PurposeAAV construct expressing eYFP-tagged chimeric opsin variant C1V1 driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_C1V1_eYFPDepositorInsertC1V1-eYFP
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2482
Plasmid#142103PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMXD3 (MXD3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo
Plasmid#170321PurposeExpresses HA and mCherry tagged RPL22, neomycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-Empty-T2A-dsRedExpress2
Plasmid#60623PurposeExpresses dsRedExpress2 in response to doxycyclineDepositorInsertEmpty
UseLentiviralTagsExpressionMutationPromoterTREAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-GFP-LacR
Plasmid#59418Purposeretroviral construct expressing the Lac repressor (LacR) tagged with GFPDepositorInsertLac repressor (LacR)
UseRetroviralTagsGFPExpressionMutationPromoterAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
canxTM-mEmerald (cytosolic)
Plasmid#186962PurposeExpresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.DepositorInsertCANX (CANX Human)
UseTagsmEmeraldExpressionMammalianMutationOnly express calnexin (482-end) and add ER signal…PromoterCMV PromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-TNFalpha
Plasmid#28089DepositorInsertTNFalpha (Tnf Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GFP(9-7)
Plasmid#165145PurposeExpresses GFP(9-7) in mammalian cellsDepositorInsertGFP(9-7)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-pegRNA-RNF2_+5GtoT
Plasmid#135957PurposeS. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editingDepositorInsertRNF2_5GtoT pegRNA
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUB1 (full-length, aa 1-271)
Plasmid#61420PurposeExpresses human OTUB1 (full-length) in E. coli.DepositorInsertOTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti 4.1 Ex miR200c-141
Plasmid#35534Purpose3rd generation lentiviral vectorDepositorUseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.16_ M4_Ef1a-gateway-V5-SV40-Zeo
Plasmid#128452Purposelentiviral destination vector with Ef1a promoter, C-terminal V5 tag, SV40 promoter, and Zeo Resistance geneDepositorInsertEf1a promoter, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF1253
Plasmid#142764PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-Puro_hCOL4A6-Myc-His
Plasmid#198087PurposeExpress hCOL4A6 in mammalian cellsDepositorInsertCOL4A6 (COL4A6 Human)
UseTagsTEV-Myc-HisExpressionMammalianMutationPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMD2-VSVGmut
Plasmid#182229PurposeExpresses VSVGmut protein in mammalian cells for lentiviral pseudotypingDepositorInsertVSVGmut
UseTagsExpressionMammalianMutationK47Q, R354APromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)
Plasmid#224123PurposeMammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)DepositorInsertPE7-mutant (Q20A_Y23A_Y24F_F35A)
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationQ20A_Y23A_Y24F_F35A in La(SSB)1-194PromoterCMVAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK1
Plasmid#111722Purposeto express human BLM protein in yeast cellsDepositorInsertBLM (BLM Human)
UseTagshexahistidineExpressionYeastMutationa C-terminal hexahistidine tag was added, a yeast…PromoterGAL1Available sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF1252
Plasmid#143778PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only