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Plasmid#89877Purposeexpresses NES-CRY2PHR-TevC of BLITz systemDepositorInsertNES-CRY2PHR-TevC
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAS9cd
Plasmid#141252PurposeConstitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterTEF1 (Cas9), pSNR52 (gRNA)Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mCherry-CyrCR1
Plasmid#128768PurposeExpresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.DepositorInsertCyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-FOXO3a WT-ER
Plasmid#8355DepositorInsertFOXO3a (FOXO3 Human)
UseTagsHA (YDVPDYASL)ExpressionMammalianMutationmouse estrogen receptor (G525R) on C-termPromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
HA-FOXO3a WT delta CT
Plasmid#1796DepositorInsertFOXO3a (FOXO3 Human)
UseTagsHAExpressionMammalianMutationdelta CT (aa468-673)PromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
HA-FOXO3a WT DBM (H212R)
Plasmid#8352DepositorInsertFOXO3a (FOXO3 Human)
UseTagsHAExpressionMammalianMutationH212RPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pALD4 T-
Plasmid#161481PurposeNon targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Non targeting control NTC)
UseTagsExpressionYeastMutationPromoterpALD4, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T-
Plasmid#161487PurposeNon targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Non targeting control NTC)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GADD45 P-2/3m-luc
Plasmid#8359DepositorInsertGADD45 promoter P-2/3m (GADD45A Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationP-2/3mPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCMV-bArrestin2-TevC-P2A-TdTomato
Plasmid#89869Purposeexpresses iTango bArrestin2-TevC component and TdTomato bicistronicallyDepositorInsertbArrestin2-TevC-P2A-TdTomato
UseSynthetic BiologyTagsExpressionMammalianMutationR173H in TdTomato (please see depositor comments …PromoterCMVAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
GADD45 P-3m-luc
Plasmid#8358DepositorInsertGADD45 promoter P-3m (GADD45A Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationP-3mPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16
Plasmid#89872Purposeexpresses CB1R-iTango component in mammalian cellsDepositorInsertCB1R-V2tail-TevN-BLITz1-TetR-VP16
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mCherry-CyrCR1.miR16
Plasmid#128747PurposeExpresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.DepositorInsertCyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB2802
Plasmid#63655PurposepCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO
Plasmid#128755PurposeExpresses CYRANO gRNA for CRISPRiDepositorInsertCYRANO sgRNA (OIP5-AS1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB2798
Plasmid#63659PurposepCfB2798 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFlag-CMV2-Brd4 FEE-AAA
Plasmid#21942DepositorInsertBRD4 (BRD4 Human)
UseTagsFlagExpressionMammalianMutationaa 1209-1362 only, FEE-AAAPromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCfB2800
Plasmid#63657PurposepCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFlag-CMV2-Brd4 R1337P
Plasmid#21939DepositorInsertBRD4 (BRD4 Human)
UseTagsFlagExpressionMammalianMutationaa 1209-1362 only, R1337PPromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFlag-CMV2-Brd4 S1351R
Plasmid#21941DepositorInsertBRD4 (BRD4 Human)
UseTagsFlagExpressionMammalianMutationaa1209-1362 only, S1351RPromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only