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pBSV2_Psyn-sgRNAftsI

Plasmid

#149632

Purpose

ftsI gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAftsI

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2B_Psyn-sgRNA500

Plasmid

#149558

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_Psyn-sgRNAflaB

Plasmid

#149560

Purpose

flaB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAflaB

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_Psyn-sgRNAftsI

Plasmid

#149562

Purpose

ftsI gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAftsI

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2H_Psyn-sgRNAftsI

Plasmid

#149563

Purpose

ftsI gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAftsI

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_Psyn-sgRNAmreB

Plasmid

#149566

Purpose

mreB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAmreB

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2H_Psyn-sgRNAmreB

Plasmid

#149567

Purpose

mreB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAmreB

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_Psyn-sgRNArodA

Plasmid

#149568

Purpose

rodA gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNArodA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA

Plasmid

#136657

Purpose

Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD9:LMG18311 chimera
sgRNA for St1Cas9
APOBEC-1 (APOBEC1 Human)
UGI
HA tag

Use

CRISPR and Synthetic Biology

Tags

2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA

Plasmid

#136654

Purpose

Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD9:CNRZ1066 chimera
sgRNA for St1Cas9
APOBEC-1 (APOBEC1 Human)
UGI
HA tag

Use

CRISPR and Synthetic Biology

Tags

2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR-nfatc1_sgRNA2

Plasmid

#132978

Purpose

nfatc1 sgRNA targeting to "5'-GTGGGAGCTCCATTGGATCG-3" in pDR274

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

nfatc1 sgRNA2 target sequence

Use

CRISPR; Sgrna generation vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR-itga9_sgRNA1

Plasmid

#132979

Purpose

itga9 sgRNA targeting to "5'-GAATGACGGAGCTCTCTACG-3" in pDR274

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

itga9 sgRNA1 target sequence

Use

CRISPR; Sgrna generation vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR-itga9_sgRNA4

Plasmid

#132980

Purpose

itga9 sgRNA targeting to "5'-TCCGCTGCCAGCCAGCCGG-3" in pDR274

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

itga9 sgRNA4 target sequence

Use

CRISPR; Sgrna generation vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR-itga9_sgRNA7

Plasmid

#132981

Purpose

itga9 sgRNA targeting to "5'-CCCTCCAGCCTTCCATATG-3" in pDR274

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

itga9 sgRNA7 target sequence

Use

CRISPR; Sgrna generation vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLR5-U6-sgRNA-EF

Plasmid

#82635

Purpose

piggyBac vector for expressing modified sgRNA in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Type

Empty backbone

Use

CRISPR; Piggybac vector

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL251 GG sgRNA

Plasmid

#126436

Purpose

To target a protospacer with GG at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with GG at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL510 CA sgRNA

Plasmid

#126430

Purpose

To target a protospacer with CA at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with CA at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL401 GC sgRNA

Plasmid

#126435

Purpose

To target a protospacer with GC at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with GC at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL071 AG sgRNA

Plasmid

#126428

Purpose

To target a protospacer with AG at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with AG at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTBL402 AC sgRNA

Plasmid

#126427

Purpose

To target a protospacer with AC at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with AC at cut site

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge