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Plasmid#42040DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationdeleted aa457-532, aa716-772 and aa804-924, F951A…PromoterCMVAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]
Plasmid#227245PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1α and pTRE3GAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-del.GW-II+III-NES-mut
Plasmid#42043DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationdeleted aa716-772 and aa804-924, F951A, F955A, I9…PromoterCMVAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-del.GW-I+III-NES-mut
Plasmid#42042DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationdeleted aa457-532 and aa804-924, F951A, F955A, I9…PromoterCMVAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα2(I)
Plasmid#119826PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagseGFPExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα1(I)
Plasmid#119843PurposeExpresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 chain (Col1a1 Mouse)
UseTagseGFPExpressionMammalianMutationCol1a1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα2(I)-L
Plasmid#120160PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagseGFPExpressionMammalianMutationCol1a2 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-LiMETER-v1
Plasmid#113933PurposeExpress an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFPDepositorInsertLOV2 and Rit polybasic domain (PB)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA3-SREBP-2-GFP (A1134-1135)
Plasmid#204203PurposeMammalian expression of SREBP-2DepositorInsertSREBP-2 (SREBF2 Human)
UseTagsT7x3 and eGFPExpressionMammalianMutationGly to Ala 1134, 1135PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
SacI ML GFP Strand 11 Short
Plasmid#164121PurposeDetect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10DepositorInsertSacI ML GFP Strand 11 Short
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
SacI ML GFP Strand 11 Long
Plasmid#164120PurposeDetect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10DepositorInsertSacI ML GFP Strand 11 Long
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_shRNA resistant PKA-Cα-mEGFP
Plasmid#114143PurposeMammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertmouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6B-(997-1084)-NES-mut
Plasmid#42025DepositorInsertTNRC6B (TNRC6B Human)
UseTagsEGFP and MycExpressionMammalianMutationcontain aa997-1084, L1009A, F1013A, M1016A, L1018APromoterCMVAvailable sinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2(WT)-sfGFP
Plasmid#145171PurposeMammalian expression plasmid for soluble ACE2-super folder GFP fusionDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-IMP2-2
Plasmid#42175DepositorInsertInsulin-like growth factor 2 mRNA binding protein 2-2 (IGF2BP2 Human)
UseTagsGFPExpressionMammalianMutationPromoterT7Available sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα2(I)-G610C
Plasmid#119827PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and GFP between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagseGFPExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tag, Gly…PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2.4-sfGFP
Plasmid#149666PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 2.4DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2-sfGFP
Plasmid#145173PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v6-sfGFP
Plasmid#145177PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 6DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only