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Plasmid#59819PurposeAllows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.DepositorInsertMps1 (TTK Human)
UseTagsEmGFPExpressionMammalianMutationKD, catalytically inactive, D664APromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXJ40 GADD34 (514-674aa)
Plasmid#75480Purposemammalian expression of GADD34 514-674 aaDepositorInsertGADD34 (PPP1R15A Human)
UseTagsFLAGExpressionMammalianMutationexpresses aa 514-674PromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXJ40 GADD34 (1-513aa)
Plasmid#75479Purposemammalian expression of GADD34 1-513 aaDepositorInsertGADD34 (PPP1R15A Human)
UseTagsFLAGExpressionMammalianMutationexpresses aa 1-513PromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXJ40 GADD34 (214-513aa)
Plasmid#78095Purposemammalian expression of GADD34 214-513 aaDepositorInsertGADD34 (PPP1R15A Human)
UseTagsFLAGExpressionMammalianMutationexpresses aa 214-513PromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Lhx2
Plasmid#74337PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Lhx2. Not for gene expression.DepositorInsertLIM homeobox protein 2 (Lhx2 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-G51PEKN
Plasmid#118582PurposeBacterial expression of E.coli DHFR G51PEKN mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationG51 mutated to P51; Glu-Lys-Asn residues inserted…PromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMK1224
Plasmid#84221PurposeLentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, BFPDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPC myc-mRAP1 I312R
Plasmid#73137PurposeExpression of myc-tagged mRAP1 with single point mutatoin I312RDepositorInsertRap1 (Terf2ip Mouse)
UseTagsmycExpressionMammalianMutationisoleucine changed to an arginine at amino acid 3…PromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-Ds
Plasmid#119069PurposeTo clone sgRNA spacer sequence for microinjections in zebrafishDepositorInsertU6a:sgRNA
UseTagsExpressionBacterialMutationPromoterZebrafish U6a promoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
TU#1806_CRISPR_unc-73_exon21
Plasmid#82360Purposeto create unc-73E null alleleDepositorInsertCas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)
UseCRISPRTagsExpressionWormMutationPromoterU6Available sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNP1047
Plasmid#222726PurposepNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cisDepositorInsertIS621 bridge RNA
UseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP2157
Plasmid#222732PurposepNP1950 - J23119-IS621 bRNA TBL-WT, DBL-WT, HDV; T7-IS621 RecombinaseDepositorInsertIS621 Helper (WT)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
M_NES 54kDa
Plasmid#201375PurposeNucleocytoplasmic transport probeDepositorInsertMAPK_NES-EGFP-4PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-ZpGal4DBD
Plasmid#199199PurposeInsert to create ZpGal4DBD knock-ins, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-ZpGal4DBD
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-p65Zp
Plasmid#199200PurposeInsert to create p65Zp knock-ins, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-p65Zp
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB511-TP-BVRA
Plasmid#203480PurposeFor Agrobacterium transformation. Rattus rattus biliverdin reductase A (BVRA) flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the 35S promoter.DepositorInsertRattus rattus biliverdin reductase A flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
L_NES 34kDa
Plasmid#201353PurposeNucleocytoplasmic transport probeDepositorInsertAdeno_NES-EGFP-1PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
L_NES 47kDa
Plasmid#201354PurposeNucleocytoplasmic transport probeDepositorInsertAdeno_NES-EGFP-3PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Diffusive 54kDa
Plasmid#201337PurposeNucleocytoplasmic transport probeDepositorInsertEGFP-4PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
H_NLS 27kDa
Plasmid#201363PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only