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pSEVA228-pro4IUPi

Plasmid

#122018

Purpose

Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.

Depositor

Gregory Stephanopoulos

Article

Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

Inserts

Choline kinase (CKI1 Budding Yeast)
Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
isopentenyl-diphosphate Delta-isomerase

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pro4

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMVA2RBS035

Plasmid

#121051

Purpose

Expresses recombinant MVA pathway in Escherichia coli

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

mvaE
mvaS
mvaK1
mvaK2
mvaD
idi

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO5d.SFFV.dCas9-KRAB.P2A.BSD

Plasmid

#90332

Purpose

CRISPR interference

Depositor

Dirk Heckl

Article

Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

Inserts

dCas9
na
SFFV
P2A-BSD

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pETM6-G6-vioABE

Plasmid

#73438

Purpose

Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

PG6-vioABE

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PG6 (orthogonal T7-lac variant)

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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155_KRAS in pmiRGlo

Plasmid

#78132

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

KRAS (KRAS Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)

Plasmid

#63139

Purpose

Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)

Depositor

Titia Sixma

Article

Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub

Inserts

Ring1b (Ring1 Mouse)
Bmi1 (Bmi1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Residues 1-109 and Residues 1-159

Promoter

tac

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
pTET-IUPi

Plasmid

#122019

Purpose

Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.

Depositor

Gregory Stephanopoulos

Article

Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

Inserts

Choline kinase (CKI1 Budding Yeast)
Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
isopentenyl-diphosphate Delta-isomerase

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pTET

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLV302

Plasmid

#119943

Purpose

Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

Depositor

Gerald Schwank

Article

Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

Insert

N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLV312.3

Plasmid

#119944

Purpose

Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

Depositor

Gerald Schwank

Article

Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

Insert

C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-Mkk7-JNK2 fusion-pcw107

Plasmid

#64618

Purpose

Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Map2k7 linker MAPK9

Use

Lentiviral

Tags

FLAG

Expression

Mutation

mouse-human fusion

Promoter

PGK

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pMVA2

Plasmid

#119951

Purpose

Expresses recombinant MVA pathway in Escherichia coli

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

mvaE
mvaS
mvaK1
mvaK2
mvaD
idi

Use

Tags

Expression

Bacterial

Mutation

A110G

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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sgMAPKAP1_1

Plasmid

#124912

Purpose

Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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sgMAPKAP1_2

Plasmid

#124911

Purpose

Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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sgMAPKAP1_3

Plasmid

#124913

Purpose

Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSS02:cyt-Peredox-mCherry

Plasmid

#161743

Purpose

cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

Depositor

Markus Schwarzländer

Article

Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

Insert

cyt-Peredox-mCherry

Use

Tags

Expression

Plant

Mutation

Promoter

Ubiquitin 10

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-hsEMX1

Plasmid

#205637

Purpose

reRNA guide targeting Homo sapiens EMX1

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-hsEMX1 (EMX1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-mScarlet-Gphn_C4a

Plasmid

#194973

Purpose

AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4a) under the control of human Synapsin promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

mScarlet-Gphn (isoform C4a) (Gphn Synthetic, Rat)

Use

AAV

Tags

mScarlet

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-mScarlet-Gphn_C4d

Plasmid

#194975

Purpose

AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4d) under the control of human Synapsin promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

mScarlet-Gphn (isoform C4d) (Gphn Synthetic, Rat)

Use

AAV

Tags

mScarlet

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-mEGFP-Gphn_P1

Plasmid

#194978

Purpose

AAV vector to drive the Flp-dependent expression of mEGFP (L221K) -Gphn (isoform P1) under the control of human Synapsin promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

mEGFP-Gphn (isoform P1) (Gphn Synthetic, Rat)

Use

AAV

Tags

mEGFP

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-mScarlet-Gphn_P1

Plasmid

#194972

Purpose

AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform P1) under the control of human Synapsin promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

mScarlet-Gphn (isoform P1) (Gphn Synthetic, Rat)

Use

AAV

Tags

mScarlet

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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