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Plasmid#169515PurposeTier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).DepositorInsertPCMV-driven dimeric red fluorescent protein TdTomato
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GFP-TAG
Plasmid#158131PurposeSingle fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)DepositorInsertsfGFP
UseTagsExpressionYeastMutationIntroduced a stop codon in place of the tyrosine …PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCORE-Hp53
Plasmid#72242PurposeContains the CORE cassette with hyg and human p53 gene markersDepositorInsertsUseTagsExpressionBacterialMutationV122A mutationPromoterAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVH232-Tier1-PmPGK1-mRuby2
Plasmid#169523PurposeTier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).DepositorInsertPPGK-driven monomeric red fluorescent protein mRuby2
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Aga1p-FAPB2.3.6
Plasmid#158147PurposeYeast display reporter for non-display yeast strains (wild-type construct)DepositorInsertAga1p-FAPB2.3.6
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
ARK2aa
Plasmid#81013PurposeE.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366)DepositorInsertsMevTo-BBa1002-pTrc-Mkco-aphA
and PMD
UseTagsExpressionMutationPromoterLacUV5 and Trc promoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbE1k-2
Plasmid#59945Purposedihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from humanDepositorInsertsdihydropterine reductase
pterin-4a-carbinolamine dehydratase
UseTagsExpressionMutationPromoterpTrcAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRC3EGFP
Plasmid#193790PurposeExpresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterPR promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas2F
Plasmid#82377PurposedCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCGDepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW012
Plasmid#83527PurposeCloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminatorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMM545-Tier1-PhCMV-Citrine
Plasmid#169505PurposeTier-1 vector encoding PhCMV-driven yellow fluorescent protein Citrine (PhCMV-Citrine-pA).DepositorInsertPCMV-driven yellow fluorescent protein citrine
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH41-Tier1-PhCMV-GLuc
Plasmid#169544PurposeTier-1 vector encoding PhCMV-driven gaussia luciferase (PhCMV-GLuc-pA)DepositorInsertPCMV-driven Gaussia Luciferase
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPC009.106
Plasmid#171142PurposeExpression of J106 scRNA on pBBR1-KmR plasmidDepositorInsertJ106 scRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas2C
Plasmid#82374PurposedCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACADepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB303-Tier1-PhCMV-TetR_VPR
Plasmid#169575PurposeTier-1 vector encoding PhCMV-driven tetracycline-dependent VPR-based transactiavtor (PhCMV-TetR-VPR-pA).DepositorInserttetracycline-controlled transactivator
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB301-Tier1-PhCMV-rTetR_VPR
Plasmid#169579PurposeTier-1 vector encoding PhCMV-driven tetracycline-activated VPR-based transactiavtor (PhCMV-rTetR-VPR-pA).DepositorInsertreverse tetracycline-controlled transactivator
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH227-Tier1-OTetO7-PCMVmin2-SEAP
Plasmid#169563PurposeTier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-2-SEAP-pA).DepositorInserttetracycline-controlled SEAP production
UseTagsExpressionMammalianMutationPromoterTetO7-PCMVminAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pChlr-7SE
Plasmid#52168PurposeEncodes module 7 with amino acids 12S-16E for recognition of nt 2GDepositorInsertPumilio homology domain amino acids 241-283 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFOX13-Tier1-PhCMV-tGFP
Plasmid#169510PurposeTier-1 vector encoding PhCMV-driven green fluorescent protein turbo GFP (PhCMV-tGFP-pA).DepositorInsertPCMV-driven turbo green fluorescent protein
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas2D
Plasmid#82375PurposedCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACGDepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only