Addgene

Showing: 461 - 480 of 5103 results

Results per page:

Sort results by:

R26-EGFP MMEJ donor vector

Plasmid

#137926

Purpose

R0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-EGFP-bpA with micro-homology arms

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Dppa1-T2A-H2B-Halo donor

Plasmid

#113118

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Dppa1 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
V954 - pDNR MCS SA no SA CmR - Donor

Plasmid

#11697

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Donor vector

Tags

Expression

Mutation

Promoter

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDonor-Prf1-mock-ires-mCherry

Plasmid

#209074

Purpose

Targeting vector for the mouse Prf1 locus to correct prf1 gene

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

Prf1-mock-ires-mCherry HDR template (parts of Prf1(S399Stop) with mutated PAM) (Prf1 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHiUGE Myc-epitope donor ORF2

Plasmid

#200376

Purpose

Tagging endogenously expressed proteins with Myc epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Myc epitope

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Y365A-GFP Donor for iMTr EED

Plasmid

#131325

Purpose

Donor to generate i-MT-r system

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

i-MT-r.Donor

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
A365Y-GFP Donor for iWTr EED

Plasmid

#131324

Purpose

Donor to generate i-WT-r system

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

i-WT-r.Donor

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZDonor-U6-myogenin stop codon gRNA

Plasmid

#69549

Purpose

Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.

Depositor

Charles Gersbach

Insert

Myogenin C terminus-targeting guide RNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDX2 ObLiGaRe Donor vector/EPB64

Plasmid

#90017

Purpose

Donor vector for ObLiGaRe/ZFN mediated targeting to CDX2 exon1 locus

Depositor

Eric-Paul Bennett

Article

Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

Insert

MCS flanked by inverted CDX2 ZFN binding sites (CDX2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ9663 - ACTB GFP-P2A CLIP Donor

Plasmid

#202688

Purpose

CLIP donor template for integrating GFP into ACTB locus. Encodes GFP with ACTB homology arms.

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

CopGFP

Use

Tags

Expression

Mutation

Promoter

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS DRR mCherry Donor EF1a BFP

Plasmid

#98896

Purpose

Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-mCherry-GFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHiUGE TurboID-HA donor (all-in-one) ORF0

Plasmid

#200383

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al bioRxiv 2022.10.06.511211

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHiUGE TurboID-HA donor (all-in-one) ORF2

Plasmid

#200385

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al bioRxiv 2022.10.06.511211

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHiUGE TurboID-HA donor (all-in-one) ORF1

Plasmid

#200384

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al bioRxiv 2022.10.06.511211

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

Plasmid

#223249

Purpose

Mammalian expression of the optimized R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
WT1-2A-eGFP PGK PuroR Donor Plasmid

Plasmid

#82333

Purpose

WT1 targeting donor

Depositor

Sean Palecek

Article

Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

Inserts

WT1 5' Arm (WT1 Human)
WT1 3' Arm (WT1 )

Use

CRISPR and TALEN

Tags

2A-eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
V308 pDNR MCS lacZ alpha - Donor

Plasmid

#11691

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Donor vector

Tags

Expression

Mutation

Promoter

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor

Plasmid

#92142

Purpose

Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

osTir1

Use

Mouse Targeting

Tags

V5 tag

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ8079 - IL2RG GFP-P2A CLIP Donor

Plasmid

#202690

Purpose

CLIP donor template for integrating GFP into IL2RG locus.

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

CopGFP

Use

Tags

Expression

Mutation

Promoter

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)

Plasmid

#85798

Purpose

donorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression system

Depositor

Roland Friedel

Article

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Insert

PPP1R12C (PPP1R12C Human)

Use

Human targeting

Tags

Expression

Mutation

homology arms for knock-in into first intron

Promoter

Available since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge