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acta2_mCherry_pA_pDestTolpA2
Plasmid#78685Purposeacta2:mCherry transgenesis constructDepositorInsertacta2 promoter (acta2 Zebrafish)
UseUnspecifiedTagsmCherryExpressionMutationPromoteracta2Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
acta2_GFP_pA_pDestTolpA2
Plasmid#78684Purposeacta2:GFP transgenesis constructDepositorInsertacta2 promoter (acta2 Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoteracta2Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
mApple-Vinculin-23
Plasmid#54961PurposeLocalization: Focal Adhesions, Excitation: 568, Emission: 592DepositorInsertVinculin (VCL Human)
UseTagsmAppleExpressionMammalianMutationV234L in Vinculin.PromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-KAT14-547-782
Plasmid#118376PurposeGateway compatible donor vector with KAT14-547-782 truncation.DepositorInsertKAT14 (KAT14 Human)
UseDonor-plasmidTagsExpressionMutationTruncation (aa 547-782)PromoterAvailable sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-KAT14-1-546
Plasmid#118377PurposeGateway compatible donor vector with KAT14-1-546 truncation.DepositorInsertKAT14 (KAT14 Human)
UseDonor-plasmidTagsExpressionMutationTruncation (aa 1-546)PromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLV-mRFP1-Human UTROPHIN_1-261
Plasmid#225052PurposeExpress in mammalian cells the mRFP1 fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.DepositorInsertHuman Utrophin residues 1-261 and RFP1 (UTRN Human, Homo Sapiens)
UseLentiviralTagsHuman UTROPHIN 1-261 and mRFP1ExpressionMammalianMutationSequence is derived from ADDGENE Plasmid #26739-R…PromoterCMV promoter and enhancerAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EGFP-Human-UTROPHIN_1-261
Plasmid#222393PurposeExpress in mammalian cells the EGFP fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.DepositorInsertHuman Utrophin residues 1-261 and EGFP (UTRN Human, Homo Sapiens)
UseLentiviralTagsEGFP and Human UTROPHIN 1-261ExpressionMammalianMutationSequence is derived from ADDGENE Plasmid #26737-G…PromoterCMV promoter and enhancerAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Lifeact EGFP
Plasmid#187686PurposeIn vivo visualization of actinDepositorInsertLifeact
UseLentiviralTagsEGFPExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Lifeact mApple
Plasmid#187687PurposeIn vivo visualization of actinDepositorInsertLifeact
UseLentiviralTagsmAppleExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Lifeact-EGFP-2XNLS
Plasmid#58467PurposeExpresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoterDepositorInsertsLifeact
2X NLS with 2X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Lifeact-tdTomato
Plasmid#64048PurposeFluorescent reporter for F-actin labeling in living cellsDepositorInsertLifeact
UseLentiviralTagstdTomatoExpressionMammalianMutationPromoterUbiquitinAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mRuby3
Plasmid#155221PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Utr261-EGFP
Plasmid#58471PurposeExpresses a cytoplasmic actin filament reporter, the tandem CH domains (a.a. 1-261) from human utrophin, on a CMV promoterDepositorInsertUtr261 (UTRN Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mCherry
Plasmid#155222PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mCitrine
Plasmid#155220PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-ECFP-Actb
Plasmid#169787PurposeAn actin FRET sensor. Must be expressed with Venus-actin for FRET.DepositorInsertActb (Actb Mouse)
UseTagsECFPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Utr230-EGFP
Plasmid#58472PurposeExpresses a cytoplasmic actin filament reporter, a truncation of the tandem CH domains (a.a. 1-230) from human utrophin, on a CMV promoterDepositorInsertUtr230 (UTRN Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBJ1807 pRS415-COF1RFP
Plasmid#37102DepositorInsertCOF1-RFP
UseTagsRFPExpressionYeastMutationSingle RFP internal insertion at Cof147T48D with …PromoterAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only