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Plasmid#188310PurposeExpression of Fre via a T7 promoter in TXTLDepositorInsertfre
UseSynthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-LuxC
Plasmid#188307PurposeExpression of LuxC via a T7 promoter in TXTLDepositorInsertluxC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-H3H
Plasmid#188303PurposeExpression of H3H via a T7 promoter in TXTLDepositorInserth3h
UseSynthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-HisGFPLuz
Plasmid#188314PurposeExpression of eGFP-Luz via a T7 promoter in TXTLDepositorInsertGFP-Luz fusion
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-LuxE
Plasmid#188309PurposeExpression of LuxE via a T7 promoter in TXTLDepositorInsertluxE
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSiMPls_N + pSiMPls_C
Plasmid#167203PurposeSiMPl plasmid pair (pSiMPls) for use with spectinomycin/streptomycinDepositorInsertsEGFP
SmR 1-75 + gp41-1 N-intein
mRuby3
gp41-1 C-intein + SmR 76-263
UseTagsExpressionBacterialMutationPromoterAmpR, CAT, pBAD, and pTrcAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF4E9D
Plasmid#185844PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>LacI>TUP1>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF9C3
Plasmid#185843PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>RevTetR_r1.7>SIN3C>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF9D91
Plasmid#185845PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>TetR>SIN3C>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFZifPZ432
Plasmid#185850PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>ZifPZ43>MED15>PAFB1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF9D93
Plasmid#185847PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>TetR>MIG1C>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF9D92
Plasmid#185846PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>TetR>TUP1>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9355(II-1_Markerfree_BackBone)
Plasmid#161597PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site II-1, (Chr II: 341622..341641)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9356(IV-1_Markerfree_BackBone)
Plasmid#161598PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site IV-1, (Chr IV: 132921..132940)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9360(IX-1_Markerfree_BackBone)
Plasmid#161601PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site IX-1, (Chr IX: 388864..388883)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9362(XV-1_Markerfree_BackBone)
Plasmid#161603PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XV-1, (Chr XV: 93186..93205)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF7E1
Plasmid#185839PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>Zif268>MED15>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF4EH4R
Plasmid#185837PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>Zif268>VP16-PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)
Plasmid#165029PurposeExpress cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherryDepositorInsertLaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterT7Available sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-LaM(N)-cpFRB1-T2A-FKBP-LaM(C)
Plasmid#165026PurposeExpress cpFRB1-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFPDepositorInsertLaM8(N)-cpFRB1-T2A-FKBP-LaM8(C)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only