Addgene

We narrowed to 20,546 results for: Tes

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SITS-ccdb-mCherry

Plasmid

#118753

Purpose

Multisite gateway destination vector for 3' tagging with mCherry for cell free expression. Parton lab clone FFF

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Tags

mCherry

Expression

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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acta1-mRuby2-VHH[GFP]

Plasmid

#163856

Purpose

Zebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRX

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

VHH[GFP]

Use

In vitro transcription of rna

Tags

Ruby2

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb

Plasmid

#141067

Purpose

Multisite gateway destination vector for cell free expression (takes two cassettes). Parton lab clone JBO

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb-EGFP

Plasmid

#118752

Purpose

Multisite gateway destination vector for 3' tagging with EGFP for cell free expression. Parton lab FEX

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Tags

EGFP

Expression

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-mCherry-ccdb

Plasmid

#118629

Purpose

Multisite gateway destination vector for 5' tagging with mCherry for cell free expression. Parton lab clone FKZ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Tags

mCherry

Expression

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-EGFP-ccdb

Plasmid

#118628

Purpose

Multisite gateway destination vector for 5' tagging with EGFP for cell free expression. Parton lab clone FEZ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Tags

EGFP

Expression

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Lifeact-mEos2-APEX2

Plasmid

#115563

Purpose

Lifeact-mEos2-APEX2 fusion for PALM and CLEM. Parton lab clone IET

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mEos2

Use

Tags

APEX2 and Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-rab10

Plasmid

#109661

Purpose

Multisite gateway vector for 3' tagging with rab10

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab10 (rab10 Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCSN071

Plasmid

#166729

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-Mxi1-NLS

Use

CRISPR

Tags

Mxi1 and SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCSN072

Plasmid

#166730

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS-Mxi1-NLS

Use

CRISPR

Tags

Mxi1 and SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab19

Plasmid

#140888

Purpose

Expression of zebrafish GFP-tagged Rab19 in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab19 (rab19 Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab26 in pCSDEST2

Plasmid

#140884

Purpose

Expression of zebrafish GFP-tagged Rab26 in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab26 (rab26 Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab40b in pCSDEST2

Plasmid

#140883

Purpose

Expression of zebrafish GFP-tagged Rab40b in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab40b (rab40b Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab13 in pCSDEST2

Plasmid

#140879

Purpose

Expression of zebrafish GFP-tagged Rab13 in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab13 (rab13 Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab38a

Plasmid

#140887

Purpose

Expression of zebrafish GFP-tagged Rab38a in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab38a (rab38a Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-CaaX(tH) in pCSDEST2

Plasmid

#140890

Purpose

Expression of GFP-CaaX (sequence from Hras) in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CaaX membrane targeting motif (H-ras)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A mmPing20F

Plasmid

#145789

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

mmPing20F

Use

Tags

Expression

Yeast

Mutation

Insertion of the FMV enhancer

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A mmPing20B

Plasmid

#145790

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

mmPing20B

Use

Tags

Expression

Yeast

Mutation

Insertion of the b-Conglycin Enhancer

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A NL60

Plasmid

#145791

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

NL60

Use

Tags

Expression

Yeast

Mutation

Insertion of the OCS Enhancer

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-Rab31

Plasmid

#109755

Purpose

Multisite gateway vector for 3' tagging with Rab31

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab31 (rab31 Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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