Addgene

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pET22-HT/prsA

Plasmid

#73624

Purpose

Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag

Depositor

James Williamson, T. J. Kappock

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

ribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-nGFPMNase

Plasmid

#187826

Purpose

express nGFPMNase protein in E coli

Depositor

Yikang Rong

Article

Stefanis et al Brain Res Mol Brain Res. 1998 Jan;53(1-2

Insert

nGFPMNASE

Use

Tags

his tag

Expression

Bacterial

Mutation

Promoter

T7 Promotor

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-mTDGa.1

Plasmid

#81051

Purpose

bacterial expression of catalytically dead murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Use

Tags

6HIS

Expression

Bacterial

Mutation

asparagine 151 changed to alanine

Promoter

T7

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pET29b-ClbP

Plasmid

#48244

Purpose

Untagged, full-length protein for in vivo peptidase assays

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP

Use

Tags

None

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-Vx3(A7)

Plasmid

#35526

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Vx3(A7)

Use

Tags

His6

Expression

Bacterial

Mutation

Changed G2 to P

Promoter

T7

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

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pET28b-NpR5600

Plasmid

#45741

Depositor

Christopher T Walsh, Emily Balskus

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

NpR5600 (Npun_R5600 Nostoc punctiforme)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pET28a-MSP1D1deltaH4/2

Plasmid

#71712

Purpose

Membrane scaffold protein (MSP), half of helix 4 truncated

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Use

Tags

6xHis and TEV site

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-TmCA-P(11)4

Plasmid

#185783

Purpose

Encodes thermostable carbonic anhydrase enzyme linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

TmCA-P(11)4

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a AHU

Plasmid

#26640

Depositor

Phoebe Rice

Article

Swinger et al EMBO J. 2003 Jul 15. 22(14):3749-60.

Insert

AHU

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pET28-mHP1alpha

Plasmid

#181911

Purpose

Bacterial protein expression vector for HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-soNadV

Plasmid

#83363

Purpose

Expresses nadV from Shewanella oneidensis in Escherichia coli

Depositor

George Cătălin Marinescu

Article

Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

Insert

nadV

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSP26

Plasmid

#199769

Purpose

Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSP26

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Promoter

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSPdH4-6

Plasmid

#199766

Purpose

Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSPdH46

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET29b-NpR5599

Plasmid

#45740

Depositor

Christopher T Walsh, Emily Balskus

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

NpR5599 (Npun_R5599 Nostoc punctiforme)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pET29a-hNABP1-C

Plasmid

#128311

Purpose

Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a Gx-NL3

Plasmid

#169108

Purpose

Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

Depositor

Maarten Merkx

Article

Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

Insert

protein G - NanoLuc - NanoLuc - NanoLuc

Use

Tags

his-tag and strep-tag

Expression

Bacterial

Mutation

Ala 24 stop

Promoter

T7

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-PU.1-ETS

Plasmid

#85734

Purpose

Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272

Depositor

Gregory Poon

Article

Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

Insert

ETS domain of murine PU.1 (residues 167-272) (Spi1 Human, Mouse)

Use

Tags

6xHis following thrombin cleavage site LVPR|G

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-hdNadV

Plasmid

#83362

Purpose

Expresses nadV from Haemophilus ducreyi in Escherichia coli

Depositor

George Cătălin Marinescu

Article

Marinescu et al Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.

Insert

nadV

Use

Tags

none

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-HsSENP6

Plasmid

#71469

Purpose

Encodes human full length SENP6 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 6 (SENP6 Human)

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET21a Hbb

Plasmid

#26639

Depositor

Phoebe Rice

Article

Mouw et al Mol Microbiol. 2007 Mar . 63(5):1319-30.

Insert

Hbb (hbb Borrelia burgdorferi)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only