Addgene

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H_NES 47kDa

Plasmid

#201348

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

HIV_NES-EGFP-3PrA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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M_NLS 47kDa

Plasmid

#201343

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP-3PrA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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H_NLS 41kDa

Plasmid

#201340

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40-EGFP-2PrA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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M_NES 41kDa

Plasmid

#201345

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

MAPK_NES-EGFP-2PrA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-TdIF2

Plasmid

#199699

Purpose

visualization of nucleolus in living mammalian cells

Depositor

Kenji Sugimoto

Article

Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

Insert

TdIF2

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCVbsd-PPARD-K421R-K422R

Plasmid

#130778

Purpose

Vector encoding PPARD cDNA for retroviral transduction

Depositor

Till Adhikary

Article

Legrand et al Nucleic Acids Res. 2019 Oct 10;47(18):95

Insert

PPARD K421R-K422R (PPARD Human)

Use

Retroviral

Tags

Expression

Mutation

K421R-K422R

Promoter

Available since

Sept. 16, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCVbsd-PPARD-T423D-T427A

Plasmid

#130792

Purpose

Vector encoding PPARD cDNA for retroviral transduction

Depositor

Till Adhikary

Article

Legrand et al Nucleic Acids Res. 2019 Oct 10;47(18):95

Insert

PPARD T423D-T427A (PPARD Human)

Use

Retroviral

Tags

Expression

Mutation

T423D-T427A

Promoter

Available since

Sept. 16, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCVbsd-PPARD-K421A-K422A

Plasmid

#130777

Purpose

Vector encoding PPARD cDNA for retroviral transduction

Depositor

Till Adhikary

Article

Legrand et al Nucleic Acids Res. 2019 Oct 10;47(18):95

Insert

PPARD K421A-K422A (PPARD Human)

Use

Retroviral

Tags

Expression

Mutation

K421A-K422A

Promoter

Available since

Oct. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCVbsd-PPARD-H413W-T427I

Plasmid

#130766

Purpose

Vector encoding PPARD cDNA for retroviral transduction

Depositor

Till Adhikary

Article

Legrand et al Nucleic Acids Res. 2019 Oct 10;47(18):95

Insert

PPARD H413W-T427I (PPARD Human)

Use

Retroviral

Tags

Expression

Mutation

H413W-T427I

Promoter

Available since

Oct. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCVbsd-PPARD-C91A-E92A

Plasmid

#130726

Purpose

Vector encoding PPARD cDNA for retroviral transduction

Depositor

Till Adhikary

Article

Legrand et al Nucleic Acids Res. 2019 Oct 10;47(18):95

Insert

PPARD C91A-E92A (PPARD Human)

Use

Retroviral

Tags

Expression

Mutation

C91A-E92A

Promoter

Available since

Oct. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCVbsd-PPARD-T423D-T427D

Plasmid

#130790

Purpose

Vector encoding PPARD cDNA for retroviral transduction

Depositor

Till Adhikary

Article

Legrand et al Nucleic Acids Res. 2019 Oct 10;47(18):95

Insert

PPARD T423D-T427D (PPARD Human)

Use

Retroviral

Tags

Expression

Mutation

T423D-T427D

Promoter

Available since

Aug. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCVbsd-PPARD-R314A-H413W

Plasmid

#130764

Purpose

Vector encoding PPARD cDNA for retroviral transduction

Depositor

Till Adhikary

Article

Legrand et al Nucleic Acids Res. 2019 Oct 10;47(18):95

Insert

PPARD R314A-H413W (PPARD Human)

Use

Retroviral

Tags

Expression

Mutation

R314A-H413W

Promoter

Available since

Aug. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCVbsd-PPARD-T261R-V279R

Plasmid

#130739

Purpose

Vector encoding PPARD cDNA for retroviral transduction

Depositor

Till Adhikary

Article

Legrand et al Nucleic Acids Res. 2019 Oct 10;47(18):95

Insert

PPARD T261R-V279R (PPARD Human)

Use

Retroviral

Tags

Expression

Mutation

T261R-V279R

Promoter

Available since

Aug. 27, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCVbsd-PPARD-H413W-K421A-K422A

Plasmid

#130765

Purpose

Vector encoding PPARD cDNA for retroviral transduction

Depositor

Till Adhikary

Article

Legrand et al Nucleic Acids Res. 2019 Oct 10;47(18):95

Insert

PPARD H413W-K421A-K422A (PPARD Human)

Use

Retroviral

Tags

Expression

Mutation

H413W-K421A-K422A

Promoter

Available since

Aug. 27, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCVbsd-PPARD-T423A-T427A

Plasmid

#130789

Purpose

Vector encoding PPARD cDNA for retroviral transduction

Depositor

Till Adhikary

Article

Legrand et al Nucleic Acids Res. 2019 Oct 10;47(18):95

Insert

PPARD T423A-T427A (PPARD Human)

Use

Retroviral

Tags

Expression

Mutation

T423A-T427A

Promoter

Available since

Aug. 27, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCVbsd-PPARD-T423A-T427D

Plasmid

#130791

Purpose

Vector encoding PPARD cDNA for retroviral transduction

Depositor

Till Adhikary

Article

Legrand et al Nucleic Acids Res. 2019 Oct 10;47(18):95

Insert

PPARD T423A-T427D (PPARD Human)

Use

Retroviral

Tags

Expression

Mutation

T423A-T427D

Promoter

Available since

Aug. 27, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mCherry-cGAS-HA

Plasmid

#231895

Purpose

Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

cGAS (CGAS Human)

Use

Tags

3xHA and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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emiRFP670-cGAS

Plasmid

#231896

Purpose

Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

cGAS (CGAS Human)

Use

Tags

emiRFP670

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRK172-α-syn-TEV-GFP

Plasmid

#166671

Purpose

Bacterial expression of alpha synuclein coupled to GFP via a TEV site for uptake studies

Depositor

Iris Lindberg

Article

Jarvela et al PLoS One. 2021 Jan 26;16(1):e0241161. do

Insert

mouse synuclein (from V. Lee) (Snca Mouse)

Use

Tags

GFP

Expression

Bacterial

Mutation

added a TEV cleavage site sequence between synucl…

Promoter

CMV

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRN3P-NLS-EGFP-deadBtgh-3NLS

Plasmid

#194470

Purpose

Encodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

Depositor

Mathieu Boulard

Article

Formichetti et al Dev Cell 10 Jan 2024

Insert

BtGH84

Use

For in vitro transcription from t3 promoter after…

Tags

SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

Expression

Mutation

Aspartic Acid 242 (in Dennis et al.) changed to A…

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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