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Plasmid#205003PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJS288
Plasmid#200714PurposeTo express yeast Hxt1 hexose transporter in yeastDepositorUseSynthetic BiologyTagsmCherryExpressionYeastMutationdomesticated for MoClo cloning (removal of intern…PromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody
Plasmid#138132PurposeExpresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertEGFP-anti-mouse-IgG(kappa)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHeEf1a-mEGFP-NLS
Plasmid#205012PurposeThe vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody
Plasmid#138128PurposeExpresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertmClover3-anti-mouse-IgG(kappa)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mTcte1-PA
Plasmid#176469PurposeExpression vector of mouse t-complex-associated testis expressed 1 (Tcte1) tagged with PA at C-terminus.DepositorInsertt-complex-associated testis expressed 1 (Tcte1 Mouse)
UseTagsPAExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-C1-IFT54
Plasmid#218726PurposeExpresses N-terminally EGFP-tagged IFT54 in mammalian cellsDepositorInsertIFT54 (TRAF3IP1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEMS-SDHB-Halo7Tag
Plasmid#108973PurposeSelflabeling tag at succinate dehydrogenase complex subunit B (SDHB)DepositorInsertsuccinate dehydrogenase complex iron sulfur subunit B (SDHB Human)
UseTagsHalo7-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGC(2.1)-U6.3
Plasmid#170516PurposePCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFPDepositorInsertgRNAcore(2.1)-pU6.3
UsePcr template vector for amplifying grnacore(2.1)-…TagsExpressionMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHeCAHS-mEGFP-NLS
Plasmid#205014PurposeThe vector contains 1kbp of the upstream and downstream regions of the chas gene (BV898_02951, CAHS 86272) from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the chas gene from H. exemplaris
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV Flag dPDZ-PTEN (1-400)
Plasmid#22232DepositorInsertPTEN (Pten Mouse)
UseTagsFlagExpressionBacterial and MammalianMutationPDZ binding domain deleted. Contains amino acids …PromoterAvailable sinceMarch 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FLJ20309
Plasmid#15360DepositorInsertFLJ20309 106-544residues (INO80D Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- WTN23C11orf83-GFP
Plasmid#65845PurposeMammalian expression of the wild type N terminal part (N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)DepositorInsertTransmembrane (23 AA) of UQCC3 (UQCC3 Human)
UseTagsGFPExpressionMammalianMutationWT TransmembranePromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
GST-Delta23C11orf83
Plasmid#65849Purposebacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)DepositorInsertUQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)
UseTagsGSTExpressionBacterialMutationdeletion of the 23 first aa (TM)PromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-IFT20
Plasmid#218719PurposeExpresses C-terminally EGFP-tagged IFT20 in mammalian cellsDepositorInsertIFT20 (IFT20 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-IFT38
Plasmid#218723PurposeExpresses N-terminally EGFP-tagged IFT38 in mammalian cellsDepositorInsertIFT38 (CLUAP1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxAmp21R
Plasmid#218287PurposeA complete plasmid for ToxAmp (Toxin-antitoxin-driven gene amplification) for the expression of AeBlueDepositorInsertHO(-955, -789)-LoxP>pKlLEU2>KlLEU2>PCRT1>RelB>tPDC1-pTDH3>AeBlue>tSYNth7-ARS712-HO(-731, -264)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxAmp1
Plasmid#218285PurposeAmplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanismDepositorInsertHO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTR(EF)-tRNA(Q)
Plasmid#170517PurposePCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80DepositorInsertgRNAcore(EF)-tRNA(Q)
UsePcr template vector for amplifying grnacore(ef)-t…TagsExpressionMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only