Addgene

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pDestattB/CY (pCM327)

Plasmid

#68341

Purpose

Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.

Depositor

Leonard Zon, Christian Mosimann

Article

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Type

Empty backbone

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pDestattB_ubi:EGFP (pCM272)

Plasmid

#68339

Purpose

Multisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.

Depositor

Leonard Zon, Christian Mosimann

Article

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Insert

EGFP

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

ubi

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pGD-γb

Plasmid

#196327

Purpose

35S promoter-driven expression of the barley stripe mosaic virus γb RNA silencing suppressor

Depositor

Zhenghe Li

Article

Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

Insert

barley stripe mosaic virus γb

Use

Tags

Expression

Plant

Mutation

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Beatrix

Plasmid

#167528

Purpose

Dual-color fluorescent reporter for the creation and detection of Cre-dependent tunable mosaics in floxed mouse lines and cells.

Depositor

Gian Michele Ratto

Article

Trovato et al Nat Commun. 2020 Dec 3;11(1):6194. doi:

Insert

Beatrix

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCBASceI

Plasmid

#26477

Purpose

I-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI site

Depositor

Maria Jasin

Article

Richardson et al Genes Dev. 1998 Dec 15. 12(24):3831-42.

Insert

pCBASceI

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pTFUbiCre

Plasmid

#139670

Purpose

Expression cassette for Cre recombinase in T-DNA vector

Depositor

James Birchler

Article

Gaeta et al Chromosoma. 2013 Jun;122(3):221-32. doi:

Insert

Cre recombinase

Use

Tags

Expression

Plant

Mutation

Promoter

Maize Ubiquitin

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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p{ie1-DsRed::GW,attB}

Plasmid

#182173

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{ie1-eGFP::GW,attB}

Plasmid

#182172

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

eGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBac{Act88F-eGFP,attB,w+}

Plasmid

#182198

Purpose

EGFP driven by Act88F enhancer

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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T7-phiC31-nos3'UTR

Plasmid

#182192

Purpose

in vitro transcription of phiC31 integrase mRNA

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

phiC31 integrase

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{3P3-mTurquoise::GW,attB}

Plasmid

#182169

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

mTurquoise

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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p{3P3-eGFP::GW,attB}

Plasmid

#182167

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{3P3-dsRed::GW,attB}

Plasmid

#182168

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{3P3-dsRed::KD,attB}

Plasmid

#182178

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBac{9Xmef2-eGFP,attB,w+}

Plasmid

#182197

Purpose

EGFP driven by MEF2 transcription factor binding sites

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBac{9Xtin-eGFP,w+,attB}

Plasmid

#182194

Purpose

EGFP driven by tinman transcription factor binding sites

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBac{3Xmef2-eGFP,attB,w+}

Plasmid

#182196

Purpose

EGFP driven by MEF2 transcription factor binding sites

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{3P3-dsRed::KD-RC,attB}

Plasmid

#182179

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBac{3Xtin-eGFP,w+,attB}

Plasmid

#182193

Purpose

EGFP driven by tinman transcription factor binding sites

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{3P3-mTurquoise::KD,attB}

Plasmid

#182182

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

mTurquoise

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge