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Plasmid#146864PurposeInsect Expression of DmPABPC1_del364-550-siRNAresDepositorInsertDmPABPC1_del364-550-siRNAres (pAbp Fly)
UseTagsExpressionInsectMutationone non silent mutation G26S, and five silent mut…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W
Plasmid#147875PurposeInsect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAresDepositorInsertDmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-L36A L69A-V5His6_Z
Plasmid#148159PurposeInsect Expression of Dm4E-T_1-511-L36AL69ADepositorInsertDm4E-T_1-511-L36AL69A (4E-T Fly)
UseTagsExpressionInsectMutationtwo non silent mutations L127S, annotated as EST,…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRedCm
Plasmid#176225PurposeLambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phageDepositorInsertLambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaB and PLAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRedCmOcr
Plasmid#176226PurposeA helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.DepositorInsertLambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaB and PLAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pREDCas9
Plasmid#71541PurposeConstitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
lambda red genes
plasmid curing system
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTP223
Plasmid#13263DepositorInsertPlac-gam-bet-exo
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pKM200
Plasmid#13079DepositorInsertPtac-gam-bet-exo operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pKM201
Plasmid#13078DepositorInsertPtac-gam-bet-exo operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pES133
Plasmid#127552PurposeVariant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13DepositorInsertF5-flanked tetracycline resistance cassette
UseTagsExpressionBacterialMutationMultiple SNPs in tetA (originally from pBR322) to…PromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACBSR-hyg
Plasmid#87830PurposeExpresses lambda-red system with hyg selection markerDepositorInserthyg
UseTagsNoneExpressionBacterialMutationNonePromoterblaAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pTC3RBSC1cI
Plasmid#193773PurposeExpresses lambda CI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertLambda CI
UseTagsExpressionBacterialMutationPromoterPLTetO-1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLC3RBSC2cI
Plasmid#193775PurposeExpresses lambda CI under the control of PLLacO-1 promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertLambda CI
UseTagsExpressionBacterialMutationPromoterPLLacO-1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas
Plasmid#62225PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRDepositorHas ServiceCloning Grade DNAInsertcas9
UseCRISPR; Lambda red recombinaseTagsExpressionBacterialMutationPromoternative cas9 promoterAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLP-hyg
Plasmid#87831PurposeExpresses FLP recombinase with hyg selection markerDepositorInserthyg
UseTagsNoneExpressionBacterialMutationNonePromoterAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only