Skip to main content
Addgene
Showing: 461 - 480 of 69253 results

  11. pLV-Blast_hCOL4A5-HA_C1567R

    Plasmid
    #198105
    Purpose
    lentiviral expression of hCOL4A5_C1567R in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A5 (COL4A5 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    C1567R
    Promoter
    EF1a
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198105-plasmid-map-sequence-id-389261

    Enlarge

  12. pLV-Blast_hCOL4A5-HA_G869R

    Plasmid
    #198104
    Purpose
    lentiviral expression of hCOL4A5_G869R in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A5 (COL4A5 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    G869R
    Promoter
    EF1a
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198104-plasmid-map-sequence-id-389260

    Enlarge

  14. Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast

    Plasmid
    #182887
    Purpose
    Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)
    Depositor
    Daniel Gerlich
    Article
    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168
    Insert
    HAUS 6 (HAUS6 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    silet mutations in aa 197 - 203 to confer resista…
    Promoter
    EF1alpha core
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182887-plasmid-map-sequence-id-367932

    Enlarge

  15. pUC19-SpRYgest_targetplasmid (KAC833)

    Plasmid
    #181748
    Purpose
    pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Type
    Empty backbone
    Use
    Substrate plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181748-plasmid-map-sequence-id-354677

    Enlarge

  16. pLKO_AU1.Ollas.V5_mCherry-NLS

    Plasmid
    #178233
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.Ollas.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178233-plasmid-map-sequence-id-352827

    Enlarge

  17. pLKO_AU1.Ollas.VSVg_mCherry-NLS

    Plasmid
    #178232
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.Ollas.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178232-plasmid-map-sequence-id-352783

    Enlarge

  18. pLKO_AU1.Ollas.S_mCherry-NLS

    Plasmid
    #178231
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.Ollas.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178231-plasmid-map-sequence-id-352782

    Enlarge

  19. pLKO_AU1.NWS.Ollas_mCherry-NLS

    Plasmid
    #178227
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.NWS.Ollas and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178227-plasmid-map-sequence-id-352777

    Enlarge

Showing: 461 - 480 of 69253 results