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Showing: 461 - 480 of 39894 results

  2. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  3. Anti-MMP9 matrix metalloproteinase-9 precursor [L51/82R]

    Plasmid
    #149455
    Purpose
    Mammalian Expression Plasmid of anti-MMP9 matrix metalloproteinase-9 precursor (Rat). Derived from hybridoma L51/82.
    Depositor
    James Trimmer
    Article
    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1
    Insert
    anti-MMP9 matrix metalloproteinase-9 precursor (Rattus norvegicus) recombinant mouse monoclonal antibody (Mmp9 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    149455-plasmid-map-sequence-id-302040

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  8. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail

    Plasmid
    #60392
    Purpose
    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) using a GAL promoter
    Depositor
    Ron Vale
    Article
    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
    Insert
    TUB1 (TUB1 Budding Yeast, Synthetic)
    Use
    Tags
    Expression
    Yeast
    Mutation
    internal 6xHis (see supplement figure 4), amino a…
    Promoter
    GAL
    Available since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. Anti-LRRK2/Dardarin kinase, C-terminus [N241A/58R]

    Plasmid
    #220411
    Purpose
    Mammalian Expression Plasmid of anti-LRRK2/Dardarin kinase, C-terminus (Human) IgG2a R-mAb. Derived from hybridoma N241A/58
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-LRRK2/Dardarin kinase, C-terminus (Homo sapiens) recombinant mouse monoclonal antibody. (LRRK2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    220411-plasmid-map-sequence-id-435564

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  15. pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 N-terminal His-Tag

    Plasmid
    #186710
    Purpose
    N-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.
    Depositor
    Paula Tamagnini
    Article
    Mascia et al Green Chemistry, 24 (2022):6156
    Insert
    alr4766
    Use
    Synthetic Biology
    Tags
    His-Tag (6His)
    Expression
    Mutation
    Promoter
    Ptrc.x.tetO2
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    186710-plasmid-map-sequence-id-368444

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  20. pcDNA3.1 Flag YFP hNKCC1 N-terminal deletion (NT53)

    Plasmid
    #49062
    Purpose
    Expresses human NKCC1 truncation mutant (aa13-222) with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    N-terminal deletion of amino acids 13-222 in hNKC…
    Promoter
    CMV
    Available since
    Nov. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    49062-plasmid-map-sequence-id-72184

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Showing: 461 - 480 of 39894 results