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Plasmid#83537PurposeConstitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genesDepositorInsertpTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas73
Plasmid#82398PurposeΔccm:Km, used to delete ccm operonDepositorInsertccm deletion
UseTagsExpressionMutationPromoternoneAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS3-aurJ
Plasmid#70152PurposeFusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector with DS3 selection markerDepositorInsertaurJ
UseTagsExpressionYeastMutationPromoterGPD1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDS2
Plasmid#70135PurposeCEN/ARS yeast shuttle vector with DS2 selection markerDepositorInsertDS2
UseSynthetic BiologyTagsExpressionMutationPromoterAgTEF1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbB0a-RFP
Plasmid#35371DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbB0k-RFP
Plasmid#35370DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbS3c-RFP
Plasmid#35311DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterProSAvailable sinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbE3c-RFP
Plasmid#35305DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterProSAvailable sinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK793
Plasmid#8827DepositorInsertTEV protease, S219V mutant
UseTagsHis, MBP (with TEV), and polyarginineExpressionBacterialMutationS219V mutation (improves stability)PromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMP11
Plasmid#215559PurposepKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 originDepositorInsertsCas9
gRNA targeting pBR322 origin of replication
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23105 and tetAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
KO plasmid
Plasmid#135970PurposeExpresses SpCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas2C
Plasmid#82374PurposedCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACADepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHB-4
Plasmid#140957PurposeFor PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.DepositorInsertphaCAB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu8
Plasmid#98297PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS1017-Tier1-OTetO7-PCMVmin-SEAP
Plasmid#169586PurposeTier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).DepositorInserttetracycline-controlled SEAP expression
UseTagsExpressionMammalianMutationPromoterTetO7-PCMVminAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Actinin-sstFRET-GR
Plasmid#83416PurposeFRET-based actinin tension sensorDepositorInsertActinin-sstFRET-GR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHB-3
Plasmid#140956PurposeFor PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.DepositorInsertphaCAB
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCK01_PalkS_alkS
Plasmid#166502PurposeExpression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertalkS
UseSynthetic BiologyTagsExpressionBacterialMutationL401GPromoterPalkSAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB_PalkB_sfgfp_KanR
Plasmid#166503PurposeExpression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPalkBAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
ARK2aa
Plasmid#81013PurposeE.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366)DepositorInsertsMevTo-BBa1002-pTrc-Mkco-aphA
and PMD
UseTagsExpressionMutationPromoterLacUV5 and Trc promoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only