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pRabies-EBFP-tTAn

Plasmid

#172120

Purpose

Expression of EBFP and tTA-InteinN in Rabies virus infected cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

EBFP, tTAn-InteinN

Use

Rabies virus

Tags

Expression

Mutation

Promoter

CMV

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.mTagBFP2.miR-ctrl

Plasmid

#169312

Purpose

Constitutive overexpression of miR-ctrl with mTagBFP2 as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-ctrl

Use

Lentiviral

Tags

TagBFP2

Expression

Mutation

Promoter

SFFV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.mTagBFP2.miR-125b-2

Plasmid

#169311

Purpose

Constitutive overexpression of miR-125b-2 with mTagBFP2 as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-125b-2 (MIR125B2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_vhhGFP4::Nrv1::tagBFP

Plasmid

#163925

Purpose

Expression of GrabFP-B-extracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor

Use

Cre/Lox

Tags

tagBFP

Expression

Insect

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAS2-his-24::tagBFP2

Plasmid

#124322

Purpose

Expressing Nuclear Localized tagBFP2

Depositor

Andrew Leifer

Insert

tagBFP2 (his-24 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 BFP-guide1

Plasmid

#118165

Purpose

CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA1 against the BFP CDS

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a core and U6

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Espin-Like-C-18

Plasmid

#62784

Purpose

Localization: Actin Bundling , C1 Cloning Vector, Excitation:399, Emission:456

Depositor

Michael Davidson

Insert

EspinLike

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

Plasmid

#102796

Purpose

Retrovirus for delivery of one sgRNA (empty, bbsI)

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

U6 sgRNA

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC13N-dCas9-BFP-KRAB

Plasmid

#127968

Purpose

constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-dCas9-NLS-BFP-KRAB

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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lenti-dCas9-VP64-BFP

Plasmid

#196711

Purpose

CRISPR-activation. dCas9-VP64 with TagBFP for sorting

Depositor

Bernhard Schmierer

Insert

dCas9-VP64-T2A-TagBFP

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mutation

D10A, N863A

Promoter

EF1a

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2p2A--3mnx1:mTagBFP-NS-MCS

Plasmid

#78250

Purpose

Can be used to subclone in gene of interest into blue motor neuron expression vector.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Inserts

-3mnx1 (mnx1 Zebrafish)
-mTagBFP-NS-MCS

Use

Zebrafish motor neuron expression

Tags

Expression

Mutation

Promoter

-3mnx1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDonor-tBFP-NLS-Neo

Plasmid

#80766

Purpose

Customizable donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Type

Empty backbone

Use

CRISPR

Tags

TagBFP2-3×NLS

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Ccdc134-IRES-BFP

Plasmid

#117266

Purpose

Retroviral overexpression of Ccdc134

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

Ccdc134 (Ccdc134 )

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA-CasRx_SD1-pSV40-TagBFP

Plasmid

#221004

Purpose

Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

Depositor

Benjamin Blencowe, Mikko Taipale

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Insert

CasRx 30nt processed direct repeat with SD1 mutation

Use

CRISPR

Tags

Expression

Mammalian

Mutation

DR1 A>T

Promoter

U6

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

Plasmid

#67974

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2ABFP cassette, WPRE

Use

Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-Cas9-gRNA-TagBFP2

Plasmid

#124774

Purpose

3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

Plasmid

#112925

Purpose

Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6 promoter, gRNA-SAM expression cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A RIGI WT

Plasmid

#167289

Purpose

Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

DDX58 (RIGI Human)

Use

Lentiviral

Tags

tagBFP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti_ABE8e-SpRY-P2A-BFP_HygroR

Plasmid

#213009

Purpose

A lentiviral vector used to create stable cell lines expressing the ABE8e-SpRY base editor and BFP

Depositor

Richard Sherwood

Article

Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

Insert

ABE8e-SpRY-D10A

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A nickase variant of SpRY

Promoter

Ef-1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

Plasmid

#67980

Purpose

Cas9 activity reporter with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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