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Plasmid#110021PurposeProtein expression and purification of HLA-DRB1_MHC-II-betaDepositorInsertHLA-DRB1_MHC-II-beta (HLA-DRB1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK211
Plasmid#110595PurposeBTK Type 6 Repressor CDS for golden gate assemblyDepositorInsertLacI reverse
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-HA-BHLHE40(S243A/E248A)
Plasmid#110155PurposeExpression of human BHLHE40 (S243A/E248A) in mammalian cellsDepositorInsertBHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
UseTagsHAExpressionMammalianMutationS243A/E248APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQC V5 MCS IRES Puro
Plasmid#110342Purposeγ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.DepositorTypeEmpty backboneUseRetroviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-SPRTN-Y117C
Plasmid#110216PurposeExpression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsFlagExpressionMammalianMutationY117C, P296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE hygro MEN1 L22R
Plasmid#11021DepositorInsertMEN1 L22R (MEN1 Human)
UseRetroviralTagsExpressionMammalianMutationL22RPromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
Calprotectin S100A9 toehold switch sensor
Plasmid#110717PurposeToehold switch sensor to detect Calprotectin S100A9 mRNA with GFP outputDepositorInsertCalprotectin S100A9 toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HRPT2 136X
Plasmid#11049DepositorInsertHRPT2 136X (CDC73 Human)
UseTagsflagExpressionMammalianMutationtruncation mutant with a stop codon introduced at…PromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
ins:MCS2; cryaa:RFP
Plasmid#110286PurposeA zebrafish insulin promoter containing vector with a second multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.DepositorTypeEmpty backboneUseZebrafishTagsExpressionMutationPromoterinsulinAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM60 FLAG NUFIP1 W40A
Plasmid#110951Purposeexpresses mammalian FLAG-NUFIP1 W40ADepositorInsertNUFIP1 W40A (NUFIP1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationchanged Tryptophan 40 to alaninePromoterCMVAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-CD5Flag2-P2A-smAID-mClover
Plasmid#110657PurposeLentiviral vector expressing cell surface Flag-tag and monomeric GFP N-terminaly fused with auxin inducible degronDepositorInsertCD5Flag2-P2A-AID-mClover
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
EYFP-Talin1-FERM
Plasmid#110565Purposebacterial expresion of EYFP-tagged Talin 1DepositorInsertTalin 1 (Tln1 Mouse)
UseTags(His)6ExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCHKU38.2-2.2
Plasmid#110670PurposeExpression of a fungal biosynthetic gene clusterDepositorInsertS_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS314-hUI
Plasmid#11006DepositorInserthUI recombination system
UseTagsExpressionBacterial and YeastMutationInverted repeat recombination system. Recombinati…PromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBTK103
Plasmid#110582PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertLac promoter with lac operator and RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKOdeltagalK (Apr)
Plasmid#110091PurposeAnalogous to pKO but without the negative selection gene galK.DepositorTypeEmpty backboneUseBacterial knockout generationTagsExpressionMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKOdeltasacB (Kan)
Plasmid#110092PurposeAnalogous to pKO but without the negative selection gene sacB.DepositorTypeEmpty backboneUseBacterial knockout generationTagsExpressionMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
hCXCR4-mTQ2
Plasmid#110196PurposeEncodes for human CXCR4 coding sequence tagged with the mTQ2 FPDepositorInserthCXCR4 (CXCR4 Human)
UseTagsmTurquoise (mTQ2)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACVR1 (ACVR1Z-c041)
Plasmid#110269PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertACVR1 (ACVR1 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
plko-tet-on-puromycin-shTRX1-259
Plasmid#110920PurposeTo knockdown Thioredoxin-1 following doxycycline additionDepositorInsertTRX1 (TXN Human)
UseLentiviralTagsExpressionMutationPromoterunknownAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only