Skip to main content
Addgene
Showing: 461 - 480 of 1021 results

  1. Tac(5A)-GFP

    Plasmid
    #162495
    Purpose
    Expresses C-terminally GFP tagged Tac with mutated five O-glycosylation sites in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    Tac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine residues are mutated into alan…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162495-plasmid-map-sequence-id-323475

    Enlarge

  2. GFP-Tac(5A)

    Plasmid
    #162493
    Purpose
    Expresses GFP tagged Tac with mutated five O-glycosylation sites in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    Tac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine residues are mutated into alan…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162493-plasmid-map-sequence-id-323471

    Enlarge

  8. SBP-GFP-Tac-TC

    Plasmid
    #162506
    Purpose
    Expresses GFP tagged partial length Tac (TMD and cytosolic tail) in RUSH vector in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    GFP and SBP tagged partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    Use
    Tags
    GFP and SBP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162506-plasmid-map-sequence-id-323518

    Enlarge

  11. CLC-EGFP206(5)

    Plasmid
    #193573
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 5 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193573-plasmid-map-sequence-id-380171

    Enlarge

  12. CLC-EGFP206(6)

    Plasmid
    #193574
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 6 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193574-plasmid-map-sequence-id-380173

    Enlarge

  13. CLC-EGFP206(7)

    Plasmid
    #193575
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 7 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193575-plasmid-map-sequence-id-383667

    Enlarge

  15. p1.1-Tr2-eGFP

    Plasmid
    #162782
    Purpose
    Fluorescent reporter for protein expression studies
    Depositor
    Ivan Vorobiev
    Article
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Insert
    enhanced green fluorescent protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    162782-plasmid-map-sequence-id-338334

    Enlarge

  17. GFP-Tac-STC

    Plasmid
    #162490
    Purpose
    Expresses partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    partial length Tac (stem region, TMD and cytosolic tail) (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its stem region, TM…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162490-plasmid-map-sequence-id-323468

    Enlarge

  18. pcDNA-GFP-EBA175-Flag

    Plasmid
    #154970
    Purpose
    Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Use
    Tags
    GFP and signal peptide (residues 1 to 32) from TG…
    Expression
    Mammalian
    Mutation
    insert codes for EBA175 residues 1284 to 1462
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154970-plasmid-map-sequence-id-308073

    Enlarge

  19. pGG-TurboID-GFP-PTS1

    Plasmid
    #209406
    Purpose
    Transiently expressing and visualizing the subcellular localization of TurboID fused with a peroxisomal targeting signal type I (PTS1) in planta
    Depositor
    Rosa Lozano-Durán
    Article
    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1
    Insert
    3XHA-TurboID-PTS1
    Use
    Tags
    GFP
    Expression
    Plant
    Mutation
    TurboID gene sequence C249A
    Promoter
    Cauliflower mosaic virus (CaMV) 35S promoter
    Available since
    Jan. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    209406-plasmid-map-sequence-id-418444

    Enlarge

  20. FKBP-LBR(1-245)-GFP

    Plasmid
    #222425
    Purpose
    To express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cells
    Depositor
    Stephen Royle
    Article
    Downie et al bioRxiv 2024.05.31.596797
    Insert
    LBR(1-245aa) (LBR Human)
    Use
    Tags
    FKBP and GFP
    Expression
    Mammalian
    Mutation
    Truncation (Includes aa 1-245)
    Promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    222425-plasmid-map-sequence-id-439254

    Enlarge

Showing: 461 - 480 of 1021 results