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Plasmid#173990PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #6 mutated
UseLuciferaseTagsExpressionMutationCoordinator motif mutation #6PromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#4
Plasmid#173988PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #4 mutated
UseLuciferaseTagsExpressionMutationCoordinator motif mutation #4PromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#7
Plasmid#173991PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #7 mutated
UseLuciferaseTagsExpressionMutationCoordinator motif mutation #7PromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_3XCoordinatorMutant#5-6-7
Plasmid#173993PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motifs #5, #6 and #7 mutated
UseLuciferaseTagsExpressionMutation3X Coordinator motif mutation #5-6-7PromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_min1-2_3XCoordinatorMutant
Plasmid#173964PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertMinimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 3 mutated Coordinator motifs
UseLuciferaseTagsExpressionMutation3 mutated Coordinator motifsPromoterSV40 promoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2 gRNA resistant
Plasmid#163408PurposeExpresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationgRNA-targeting sequences are substituted with nuc…PromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔEC gRNA resistant
Plasmid#163413PurposeExpresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe extracellular domain (27-150 aa) is deleted. …PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔTail gRNA resistant
Plasmid#163412PurposeExpresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe C-terminal tail domain (530-1038 aa) is delet…PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (E143amb)-His
Plasmid#168046PurposeExpresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationGlu 143 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (S87amb)-His
Plasmid#168044PurposeExpresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationSer 87 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N116amb)-His
Plasmid#168045PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationAsn 116 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N21amb)-His
Plasmid#168041PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationAsn 21 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-T2A-mCherry pDRF1-GW
Plasmid#168060PurposeProduces equimolar expression of mGreenLantern and mCherry for use in yeastDepositorInsertmGreenLantern-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (I55amb)-His
Plasmid#168042PurposeExpresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationIle 55 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 AURKA deltaNter
Plasmid#157749PurposeExpression of AURKA without Nter in mammalian cellsDepositorInsertAURKA (AURKA Human)
UseTagsExpressionMammalianMutationthe first 30AA of Aurora A are lackingPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Anti-ZNF423 [N328B/37R]
Plasmid#140077PurposeMammalian Expression Plasmid of anti-ZNF423 (Human). Derived from hybridoma N328B/37.DepositorInsertanti-ZNF423 (Human) recombinant mouse monoclonal antibody (ZNF423 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-Kv1.5 K+ channel [K7/45R]
Plasmid#140069PurposeMammalian Expression Plasmid of anti-Kv1.5 K+ channel (Rat). Derived from hybridoma K7/45.DepositorInsertanti-Kv1.5 K+ channel (Rat) recombinant mouse monoclonal antibody (Kcna5 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-DNMT3L [N117/9.5R]
Plasmid#114514PurposeMammalian Expression Plasmid of anti-DNMT3L (Human). Derived from hybridoma N117/9.5.DepositorInsertanti-DNMT3L (Homo sapiens) recombinant mouse monoclonal antibody (DNMT3L Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4-eIF3B MBE3 mutant
Plasmid#133310PurposeExpression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3DepositorInserteIF3B promoter MBE3 mutant (EIF3B Human)
UseLuciferaseTagsExpressionMammalianMutationccacgtgacc changed to cAaAAAAaccPromotereIF3BAvailable sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4-eIF3B MBE2 mutant
Plasmid#133309PurposeExpression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2DepositorInserteIF3B promoter MBE2 mutant (EIF3B Human)
UseLuciferaseTagsExpressionMammalianMutationgccacatgcacc changed to gcAaAaAAcaccPromotereIF3BAvailable sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-DLX1 [L43/40R]
Plasmid#128633PurposeMammalian Expression Plasmid of anti-DLX1 (Human). Derived from hybridoma L43/40.DepositorInsertanti-DLX1 (Human) recombinant mouse monoclonal antibody (DLX1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-REEP1/2 [N326D/29R]
Plasmid#114510PurposeMammalian Expression Plasmid of anti-REEP1/2 (Mouse). Derived from hybridoma N326D/29.DepositorInsertanti-REEP1/2 (Mus musculus) recombinant mouse monoclonal antibody (Reep1 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_shRNA resistant nmPKA-Cα-mEGFP
Plasmid#114144PurposeThe G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertmouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
UseTagsExpressionMammalianMutationG2A mutation and silent mutation to resist shRNA …PromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Anti-MESD [N88/12.1R]
Plasmid#114539PurposeMammalian Expression Plasmid of anti-MESD (Mouse). Derived from hybridoma N88/12.1.DepositorInsertanti-MESD (Mus musculus) recombinant mouse monoclonal antibody (Mesd Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Anti-Malin [N85/18.1R]
Plasmid#114513PurposeMammalian Expression Plasmid of anti-Malin (Human). Derived from hybridoma N85/18.1.DepositorInsertanti-Malin (Homo sapiens) recombinant mouse monoclonal antibody (NHLRC1 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Anti-Botch [N116/14R]
Plasmid#114487PurposeMammalian Expression Plasmid of anti-Botch (Rat). Derived from hybridoma N116/14.DepositorInsertanti-Botch (Rattus norvegicus) recombinant mouse monoclonal antibody (Chac1 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT9 deltaTPR mutant
Plasmid#67614Purposebacterial expression of GST-PRMT9 missing residues 21-139DepositorInsertPRMT9 (PRMT9 Human)
UseTagsGSTExpressionBacterialMutationtruncation mutant missing aa 21-139PromotertacAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1 SADBs shRNA Resistant
Plasmid#67155PurposepEGFP containing N-terminally EGFP Tagged mouse SADBs resistant to an shRNADepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsEGFPExpressionMammalianMutationnucleotide 294 C-T, nucleotide 297 C-GPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5 N-HA SADBs shRNA resistant
Plasmid#67157PurposeN-terminally HA tagged mouse SADBs containing substitutions providing resistance to an shRNADepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsHAExpressionMammalianMutationNucleotide 294 C-T Nucleotide 297 C-G (shRNA resi…PromoterSV40 promoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
His-tagged-SF1 (antisense)
Plasmid#28183DepositorInsertSF1 (Nr5a1 Mouse)
UseTagsHisExpressionMammalianMutationReverse OrientationPromoterAvailable sinceMay 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
CJ47 (U1 snRNA (U1;21d) 3G mutant)
Plasmid#17776DepositorInsertU1 snRNA (U1;21d) (snRNA:U1:21D Fly)
UseTagsExpressionMutation3G mutantPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
XE154 XDpr-pt mutant-CS2+
Plasmid#16768DepositorInsertXDpr-pt mutant (dact1-b Frog)
UseXenopus expressionTagsExpressionMutationlast four amino acids mutated to MNTV instead of …PromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2alpha D291N mutant
Plasmid#128813PurposeTo make virus expressing Doc2alpha D291 mutantDepositorInsertDoc2alpha (Doc2a Rat)
UseLentiviralTagsIRES-EGFPExpressionMutationD291NPromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2beta D303N mutant
Plasmid#128815PurposeTo make virus expressing Doc2beta D303N mutantDepositorInsertDoc2beta (Doc2b Rat)
UseLentiviralTagsIRES-EGFPExpressionMutationD303NPromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
HIP-flash (HOP-flash mutant)
Plasmid#83466PurposeNegative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter geneDepositorInsertMultimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Anti-CASPR/Neurexin IV [K65/35R]
Plasmid#177445PurposeMammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat). Derived from hybridoma K65/35.DepositorInsertanti-CASPR/Neurexin IV (Rattus norvegicus) recombinant mouse monoclonal antibody (Cntnap1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
M51 Super 8x FOPFlash (TOPFlash mutant)
Plasmid#12457PurposeControl for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.DepositorInsertmutant TCF/LEF binding sites (Ctnnb1 )
UseLuciferaseTagsExpressionMammalianMutationmutant TCF/LEF binding sitesPromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET29-bdSUMO-Anti-ALFA nanobody
Plasmid#212311PurposeExpression of anti-ALFA nanobodyDepositorInsertanti-ALFA nanobody
UseTagsbdSUMOExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-PDL1(KN035) PAGER(TF)
Plasmid#230003PurposeExpresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-Traditional CAR (anti-CD19)
Plasmid#192115PurposeLentiviral delivery of Traditional CAR(anti-CD19)DepositorInsertpHR-pEF1a-Traditional CAR (anti-CD19scFV_CD28_41BB_CD3z)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only