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MSCV-Puro-FH-AGO2-S828E

Plasmid

#91995

Purpose

Expresses FLAG-HA-AGO2 (S828E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828E

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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PD2529 human alpha5 ecto

Plasmid

#221394

Purpose

Mammalian expression of human integrin alpha5 ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin alpha5 ectodomain (ITGA5 Human)

Use

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HR…

Expression

Mammalian

Mutation

codon optimized for human mature residues _5 F1 t…

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DDX43

Plasmid

#134574

Purpose

Express human DDX43 protein (WT, full-length) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43 (DDX43 Human)

Use

Tags

His

Expression

Bacterial

Mutation

full-length WT

Promoter

T7

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-hCD59-T2A-GFP

Plasmid

#205460

Purpose

lentiviral plasmid for expression of human CD59

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD59 (CD59 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

UBC

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_FH-AGO2-5xA

Plasmid

#92007

Purpose

Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824A, S828A, T830A, S831A, S834A (5xA)

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_FH-AGO2-5xE

Plasmid

#92008

Purpose

Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E (5xE)

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-DDX43-K292A

Plasmid

#134575

Purpose

Express human DDX43 mutant (K292A, ATPase dead) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43-K292A (DDX43 Human)

Use

Tags

His

Expression

Bacterial

Mutation

full-length (K292A)

Promoter

T7

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast FLVCR1b-3X FLAG

Plasmid

#218536

Purpose

human FLVCR1b with C-terminal 3X_FLAG cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

Retroviral

Tags

FLAG (3X)

Expression

Mammalian

Mutation

Alternative isoform

Promoter

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast FLVCR1/FLVCR1a-3X FLAG

Plasmid

#218534

Purpose

human FLVCR1/FLVCR1a with C-terminal 3x-FLAG cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

Retroviral

Tags

FLAG (3X)

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824E-S828E

Plasmid

#91999

Purpose

Expresses FLAG-HA-AGO2 (S824E-S828E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Puro-FH-AGO2-S824E-S831E

Plasmid

#92000

Purpose

Expresses FLAG-HA-AGO2 (S824E-S831E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S831E

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Puro-FH-AGO2-S828E-S831E

Plasmid

#92001

Purpose

Expresses FLAG-HA-AGO2 (S828E-S831E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828E, S831E

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Puro-FH-AGO2-S824E-S828E-S831E

Plasmid

#92002

Purpose

Expresses FLAG-HA-AGO2 (S824E-S828E-S831E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E, S831E

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Puro-FH-AGO2-S824A-T830A

Plasmid

#92003

Purpose

Expresses FLAG-HA-AGO2 (S824A-T830A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824A, T830A

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-DDX43-FL-G84D

Plasmid

#134577

Purpose

Express human DDX43 mutant (full-length with G84D mutation) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43-G84D (DDX43 Human)

Use

Tags

His

Expression

Bacterial

Mutation

full-length (G84D)

Promoter

T7

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-HRAS(G12V)-IRES-p53DD

Plasmid

#233185

Purpose

Retroviral expression of HRAS(G12V) and p53DD (RIDD)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

HRAS(G12V)-IRES-p53DD (HRAS, Trp53 Mouse, Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-SNAP-p53DD-IRES-HRAS(G12V)

Plasmid

#233201

Purpose

Gateway entry vector for SNAP-p53DD and HRAS(G12V)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

SNAP-p53DD-IRES-HRAS(G12V) (Trp53, HRAS Mouse, Human)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-HRAS(G12V)-IRES-p53DD

Plasmid

#233186

Purpose

Gateway entry vector for HRAS(G12V) and p53DD (RIDD)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

HRAS(G12V)-IRES-p53DD (HRAS, Trp53 Mouse, Human)

Use

Other

Tags

Expression

Mutation

G12V

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-p53DD-IRES-HRAS(G12V)

Plasmid

#233187

Purpose

Retroviral expression of p53DD and HRAS(G12V) (DDIR)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

p53DD-IRES-HRAS(G12V) (Trp53, HRAS Mouse, Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-p53DD-IRES-HRAS(G12V)

Plasmid

#233188

Purpose

Gateway entry vector for p53DD and HRAS(G12V) (DDIR)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

p53DD-IRES-HRAS(G12V) (Trp53, HRAS Mouse, Human)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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