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pEN_TmiR_G12-E

Plasmid

#25761

Purpose

Entry vector with TRE promoter driving mouse G alpha 12 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-RhoA*

Plasmid

#198716

Purpose

Expresses gRNA resistant RhoA under the CAG promoter in mammalian cells

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoA (Rhoa Mouse)

Use

Tags

Expression

Mammalian

Mutation

It has mutations that is resistant to the RhoA gR…

Promoter

CAG

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-ARHGAP45

Plasmid

#221301

Purpose

Expression of ARHGAP45 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

ARHGAP45 (pLxIS) (Hmha1 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23_Promoter_4

Plasmid

#210630

Purpose

Luciferase experiment for TAL1 promoter 4

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

promoter 4 (TAL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Minimal Promoter

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23_Promoter_5

Plasmid

#210632

Purpose

Luciferase experiment for TAL1 promoter 5

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

promoter 5 (TAL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Minimal Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23_exon 4

Plasmid

#210631

Purpose

Luciferase experiment for TAL1 exon 4

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 exon 4 (TAL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Minimal Promoter

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_Ex3_1

Plasmid

#210625

Purpose

gRNA target sequences for TAL1 Exon 3

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 Exon 3 gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_2

Plasmid

#210622

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_1

Plasmid

#210621

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_4

Plasmid

#210624

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_3

Plasmid

#210623

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_Ex3_2

Plasmid

#210626

Purpose

gRNA target sequences for TAL1 Exon 3

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 Exon 3 gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_Ex3_3

Plasmid

#210627

Purpose

gRNA target sequences for TAL1 Exon 3

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 Exon 3 gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_Ex3_4

Plasmid

#210628

Purpose

gRNA target sequences for TAL1 Exon 3

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 Exon 3 gRNA (TAL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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MIGR1_FLAG_TAL1-short_GFP

Plasmid

#210637

Purpose

Overexpression of TAL1-short

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1-short (TAL1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.2 DomI + DomII pcDNA3

Plasmid

#206094

Purpose

expression of the N-terminus, Domain I, Domain II and the II-III loop (amino acids 1-1154) of rabbit Cav2.2 calcium channel

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Bn CAAX pcDNA3

Plasmid

#206088

Purpose

Cav2.2 N-terminus (amino acids 1-95) with a C-terminal tag of the final 10 aa of H.Ras containing CAAX motif

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 pCAGGS

Plasmid

#206101

Purpose

expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 W391A pRK5

Plasmid

#206084

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 DomI pMT2

Plasmid

#206127

Purpose

expression of the N-terminus, Domain I and the I-II loop (amino acids 1-483) of rabbit Cav2.2 calcium channel

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ad MLP/TPL/SV40

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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