Addgene

We narrowed to 15,717 results for: puromycin

Showing: 4761 - 4780 of 15717 results

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SRSF2 A9.3 gRNA

Plasmid

#90902

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRSF2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SRSF2 (Guide Designation A9.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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SRSF2 A10.3 gRNA

Plasmid

#90903

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRSF2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SRSF2 (Guide Designation A10.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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LIN52 D5.4 gRNA

Plasmid

#90734

Purpose

3rd generation lentiviral gRNA plasmid targeting human LIN52

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

LIN52 (Guide Designation D5.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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sgRNA3_MYOD1

Plasmid

#64138

Purpose

Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_MYOD1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pSUPER-CXCR4

Plasmid

#42052

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

CXCR4 shRNA (CXCR4 )

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pLJM60 FLAG NUFIP1 W40A

Plasmid

#110951

Purpose

expresses mammalian FLAG-NUFIP1 W40A

Depositor

David Sabatini

Article

Wyant et al Science. 2018 May 18;360(6390):751-758.

Insert

NUFIP1 W40A (NUFIP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed Tryptophan 40 to alanine

Promoter

CMV

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aalb_726

Plasmid

#176661

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aaeg_763

Plasmid

#176658

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLPC-NFLAG TRF1 deltaA deltaM

Plasmid

#16065

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Deleted acidic domain (aa1-66) and myb domain (aa…

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
TUBB F2.4 gRNA

Plasmid

#90925

Purpose

3rd generation lentiviral gRNA plasmid targeting human TUBB

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TUBB (Guide Designation F2.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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p8141 MSCV-P C-FlagHA 18E7 D10K

Plasmid

#163306

Purpose

Expresses HPV18 E7 D10K

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV18 E7 D10K

Use

Retroviral

Tags

FlagHA

Expression

Mutation

D10K

Promoter

MSCV LTR

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Non-targeting CRISPRi plasmid

Plasmid

#154891

Purpose

Human non-targeting CRISPRi gRNA

Depositor

Joseph Dougherty, Rob Mitra

Article

Lalli et al Genome Res. 2020 Sep;30(9):1317-1331. do

Insert

Nontargeting gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE HSF Flag Ac

Plasmid

#1949

Depositor

Robert Kingston

Article

Sullivan et al Mol Cell Biol 2001 Sep;21(17):5826-37.

Insert

Heat Shock Factor 1 (HSF1 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

Acidic mutations (D416, E493, E496 A)

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
GG-EBNA-TdT-guide1-PGK-Puro

Plasmid

#102903

Purpose

EBNA episome plasmid for U6 promoter-driven expression of a control gRNA targeting TdTomato sequence. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

TdT-guide1-PGK-Puro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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p6900 PHAGE-P CMVT N-HA-hE6AP III C840A

Plasmid

#37606

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP III C840A (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C840A

Promoter

CMV tet regulatable

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
hPlekhm1-C/pIRES puro Glue (S778-A1056)

Plasmid

#73456

Purpose

Express human C-terminal part (Q778-A1056) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1-N/pIRES puro Glue (M1-A497)

Plasmid

#73454

Purpose

Express human N-terminal part (M1-A497) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, Ha tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
DKC1 F1.3 gRNA

Plasmid

#90652

Purpose

3rd generation lentiviral gRNA plasmid targeting human DKC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DKC1 (Guide Designation F1.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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p6869 pOZ-N puro HA-HPV18 E2

Plasmid

#35134

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV18 E2 (E2 HPV18)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
pLG1-puro-sgATL3-2

Plasmid

#109010

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-sgRNA #2 (ATL3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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