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pDONR223-MAP2K1IP1

Plasmid

#23694

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP2K1IP1 (LAMTOR3 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DAK

Plasmid

#23870

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DAK (DAK Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pYY166 pcDNA3.4-His-MBP-hAID

Plasmid

#231942

Purpose

Expression of His-MBP-AID fusion protein in mammalian cells

Depositor

Fei-Long Meng

Article

Wang et al Cell. 2023 May 11;186(10):2193-2207.e19.

Insert

AID (AICDA Human)

Use

Tags

His-MBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hAP1 420-440

Plasmid

#183643

Purpose

To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

ATP6AP1 (ATP6AP1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

truncated ATP6AP1 mutant; contains only the trans…

Promoter

CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RPS14(89-151)

Plasmid

#122299

Purpose

expresses a part of RPS14 protein with a flag tag

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RPS14 (RPS14 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1 to 88

Promoter

CMV

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RPS14(2-88)

Plasmid

#122298

Purpose

expresses a part of RPS14 protein with a flag tag

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RPS14 (partial) (RPS14 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 89 to 151

Promoter

CMV

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-GK2

Plasmid

#23827

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GK2 (GK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CDKL4

Plasmid

#23382

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDKL4 (CDKL4 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DGKB

Plasmid

#23477

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DGKB (DGKB Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-GK5

Plasmid

#23554

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GK5 (GK5 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DCAKD

Plasmid

#23585

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DCAKD (DCAKD Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-GLYCTK

Plasmid

#23653

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GLYCTK (GLYCTK Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-LRPPRC

Plasmid

#23521

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LRPPRC (LRPPRC Human)

Use

Gateway donor vector

Tags

Expression

Mutation

G246A; deleted E579

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro

Plasmid

#167832

Purpose

Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

HRAS(N17) (HRAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S17N

Promoter

Ubc, TRE

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pT3EF1aH-myr-Akt

Plasmid

#179909

Purpose

This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

Depositor

Xin Chen

Article

Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

Insert

Akt1 (Akt1 Mouse)

Use

Tags

HA and Myr

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-BMPR2

Plasmid

#23669

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BMPR2 (BMPR2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-BUB1B

Plasmid

#23858

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BUB1B (BUB1B Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-ABL1

Plasmid

#23939

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ABL1 (ABL1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-CSF1R

Plasmid

#23928

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSF1R (CSF1R Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-NEK2

Plasmid

#23658

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK2 (NEK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Insert encodes truncated protein relative to NP_0…

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only