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Plasmid#222656PurposeMammalian expression vector to express full-length Regnase-3 tagged with FLAG at N-term and HA at C-termDepositorInsertZc3h12c (Zc3h12c Mouse)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-FLAG-Regnase-3_1-547 aa
Plasmid#222657PurposeMammalian expression vector to express 1-547 aa of Regnase-3 tagged with FLAG at N-termDepositorInsertZc3h12c 1-547 aa (Zc3h12c Mouse)
UseTagsFLAGExpressionMammalianMutationmutated histidine 548 to a stop codonPromoterCMVAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-FLAG-Regnase-3_1-409 aa
Plasmid#222658PurposeMammalian expression vector to express 1-409 aa of Regnase-3 tagged with FLAG at N-termDepositorInsertZc3h12c 1-409 aa (Zc3h12c Mouse)
UseTagsFLAGExpressionMammalianMutationmutated proline 410 to a stop codonPromoterCMVAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-FLAG-Regnase-3_1-244 aa
Plasmid#222659PurposeMammalian expression vector to express 1-244 aa of Regnase-3 tagged with FLAG at N-termDepositorInsertZc3h12c 1-244 aa (Zc3h12c Mouse)
UseTagsFLAGExpressionMammalianMutationmutated asparagine 245 to a stop codonPromoterCMVAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-FLAG-human cGASdeltaN-GFP
Plasmid#186879PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal GFP fusion (Adamts1 Synthetic, Human)
UseRetroviralTagsFLAG and GFPExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pScalps-Puro-FLAG-HA-Regnase-3 D252N
Plasmid#222664PurposeLentiviral vector to express mouse Regnase-3 D252N mutant tagged with FLAG-HA at N-termDepositorInsertZc3h12c (Zc3h12c Mouse)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationmutated aspartic acid 252 to asparagine to abroga…PromoterSFFVAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57 mini-FLAG-HA-Regnase-3 D252N
Plasmid#222652PurposeFor in vitro transcription of mouse Zc3h12c D252N mutant tagged with FLAG-HA at N-termDepositorInsertZc3h12c (Zc3h12c Mouse)
UseIn vitro transcriptionTagsFLAG and HAExpressionMutationmutated aspartic acid 252 to asparagine to abroga…PromoterT7Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEXP(FLAG/HA-NSP1-K164-H165A)
Plasmid#188782PurposeExpresses FLAG/HA-NSP1-K164-H165A in mammalian cells (SARS-CoV-2 mutant NSP1)DepositorInsertFLAG/HA-NSP1-K164-H165A (SARS-CoV-2)
UseFrt site to insert into hek293 t-rex genomeTagsFLAG/HAExpressionMammalianMutationChanged Lysine 164 to Alanine and Histidine 165 t…PromoterCMV Dox inducibleAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRC1 CTFdeltaTA-FLAG
Plasmid#217717PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertCELSR1 (CELSR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2453PromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG7 CTF-FLAG
Plasmid#217705PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGR7 (ADGRG7 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-415PromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRB2 CTFdeltaTA-FLAG
Plasmid#217715PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRB2 (BAI2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-917PromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRA3 CTFdeltaTA-FLAG
Plasmid#217713PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRA3 (ADGRA3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-743PromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast FLVCR1b-3X FLAG
Plasmid#218536Purposehuman FLVCR1b with C-terminal 3X_FLAG cDNADepositorInsertFLVCR1 (FLVCR1 Human)
UseRetroviralTagsFLAG (3X)ExpressionMammalianMutationAlternative isoformPromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-HsPcdh21-Myc-Flag-His
Plasmid#210818PurposeMammalian overexpression vector for C-terminally Myc and Flag and His tagged human Pchd21 (WT)DepositorInsertProtocadherin 21 (CDHR1 Human)
UseTagsMyc, 3x Flag, 10x HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD
Plasmid#169818PurposeExpresses C-terminal flag-tagged CAD in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationTCCC -> AGTC silent mutations at nt527-530 eli…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-APEX2-GFP-3xFLAG-PuroR
Plasmid#221238PurposeCRISPaint gene taggingDepositorInsertAPEX2-GFP-3xFLAG
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag/HA-TRBP-L326A/Y358A
Plasmid#215892PurposeExpression of TRBP-L326A/Y358ADepositorInsertTRBP-L326A/Y358A (TARBP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/T0-3XFLAG-NESm-NFATC2IP
Plasmid#221445PurposeMammalian expression vector of triple Flag tagged NFATC2IP ORF with mutant nuclear export signal (NESm) at N-terminusDepositorInsertNFATC2IP (NFATC2IP Human)
UseTags3XFlag and Mutant Nuclear Export Signal (mNES)ExpressionMammalianMutationmutant NES (NESm: NLVDLQKKLEEAKADEQQ (PMID: 10739…PromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-3XFLAG-NFATC2IP SLD2
Plasmid#221388PurposeMammalian expression vector of triple Flag tagged NFATC2IP ORF SUMO-like domain 2 (SLD2, aa 345-418)DepositorInsertNFATC2IP (NFATC2IP Human)
UseTags3XFlagExpressionMammalianMutationAmino acids 1-344 of NFATC2IP deletedPromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-3XFLAG-SLD1+SLD2
Plasmid#221389PurposeMammalian expression vector of triple Flag tagged NFATC2IP ORF SUMO-like domain 1 and SUMO-like domain 2 (SLD1+SLD2, aa 251-419)DepositorInsertNFATC2IP (NFATC2IP Human)
UseTags3XFlagExpressionMammalianMutationAmino acids 1-250 of NFATC2IP deletedPromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits