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pTag-RFP-C-h-Rab5a-c-Myc

Plasmid

#79802

Purpose

Express TagRFP labeled human Rab5a (GTPase located on early endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab5a (RAB5A Human)

Use

Tags

TagRFP and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

Plasmid

#162333

Purpose

Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

dSpCas9-2xVP64

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-SV40 polyA signal

Plasmid

#48627

Purpose

Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.

Depositor

Tomas Santalucia, Anna Planas

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

SV40 early polyadenylation signal cassette

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

none

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pTet-O-NEUROG3-T2A-PuroR

Plasmid

#162341

Purpose

Lentiviral expression of NEUROG3 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

NEUROG3 (NEUROG3 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-eArch 3.0-EYFP

Plasmid

#35514

Purpose

Lentiviral expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

eArch3.0-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pSG5 EDD

Plasmid

#37191

Depositor

Darren Saunders, Charles Watts

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD (UBR5 Human)

Use

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

K503R (please see depositor comments below)

Promoter

SV40

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(276-598)

Plasmid

#135437

Purpose

Expresses SFPQ 276-598 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

Splicing Factor Proline And Glutamine Rich (SFPQ Human)

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV sMTase (eGFP)

Plasmid

#89930

Purpose

Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.

Depositor

Carl Novina

Article

Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

Insert

dCas9-MC-IRES-MN

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

deactivated Cas9

Promoter

CMV

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe(puro) mouse Egr3

Plasmid

#108102

Purpose

Retroviral vector for expression of mouse Egr3

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr3 (Egr3 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTet-O-OVOL1-T2A-PuroR

Plasmid

#162347

Purpose

Lentiviral expression of OVOL1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

OVOL1 (OVOL1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-Mac 3.0-EYFP

Plasmid

#35515

Purpose

Lentiviral expression of CaMKIIa-driven Mac 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

eMac 3.0-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Trafficking signal, ER export

Promoter

CaMKIIa

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pOTTC840 - pAAV 5xATF6 secNLuc

Plasmid

#82497

Purpose

Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

Depositor

Brandon Harvey

Article

Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

Insert

secNLuc

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

5x(ATF6 binding site) c-Fos minimal promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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UBQLN2_450-624W

Plasmid

#176852

Purpose

Expresses human UBQLN2 construct 450-624 with added C-terminal W in bacterial cells

Depositor

Carlos Castañeda

Article

Dao et al Mol Cell. 2018 Mar 15;69(6):965-978.e6.

Insert

UBQLN2_450C_W (UBQLN2 Human)

Use

Tags

Expression

Bacterial

Mutation

deletion of residues 1-449 and addition of C-term…

Promoter

T7

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe(puro) mouse Egr4

Plasmid

#108105

Purpose

Retroviral vector for expression of mouse Egr4

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr4 (Egr4 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 AnxA6

Plasmid

#20407

Depositor

Carlos Enrich

Article

Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

Insert

AnxA6 (Anxa6 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2009

Availability

Academic Institutions and Nonprofits only
pDEST-EGFP-MACROD1

Plasmid

#172584

Purpose

For transient expression of N-terminal GFP-tagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP-MACROD2

Plasmid

#172585

Purpose

For transient expression of N-terminal GFP-tagged MACROD2

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD2 (MACROD2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pSirenRetroQ(puro)-shEgr3

Plasmid

#108115

Purpose

pSIREN-RetroQ vector containing shRNA sequence to Egr3

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

shRNA to Egr3 (Egr3 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCasper-pNos-PCP-eGFP

Plasmid

#180274

Purpose

to epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)

Depositor

Hernan Garcia

Article

Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

Insert

PCP-eGFP

Use

Tags

eGFP

Expression

Insect

Mutation

Promoter

nanos

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse Egr4

Plasmid

#108096

Purpose

Expresses mouse Egr4 in mammalian cells

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr4 (Egr4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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